Anti-Liver Arginase antibody (ab92274)
Key features and details
- Goat polyclonal to Liver Arginase
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-Liver Arginase antibody
See all Liver Arginase primary antibodies -
Description
Goat polyclonal to Liver Arginase -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Pig
Predicted to work with: Rabbit, Cow, Dog -
Immunogen
Synthetic peptide:
CFGLAREGNHKPID
, corresponding to C terminal amino acids 303-316 of Human liver Arginase (NP_000036.2). -
Positive control
- WB: Pig, mouse, human and rat liver lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92274 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Pathway
Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1. -
Involvement in disease
Defects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia. -
Sequence similarities
Belongs to the arginase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 383 Human
- Entrez Gene: 11846 Mouse
- Entrez Gene: 29221 Rat
- Omim: 608313 Human
- SwissProt: P05089 Human
- SwissProt: Q61176 Mouse
- SwissProt: P07824 Rat
- Unigene: 440934 Human
see all -
Alternative names
- A I antibody
- Al antibody
- ARG 1 antibody
see all
Images
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All lanes : Anti-Liver Arginase antibody (ab92274) at 0.03 µg/ml
Lane 1 : pig liver tissue lysate
Lane 2 : mouse liver tissue lysate
Lane 3 : rat liver tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 35 kDa
Observed band size: 35 kDa -
Anti-Liver Arginase antibody (ab92274) at 0.01 µg/ml + human liver lysate in RIPA buffer at 35 µg
Predicted band size: 35 kDa
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab92274 has been referenced in 3 publications.
- Han GH et al. Transplantation of tauroursodeoxycholic acid-inducing M2-phenotype macrophages promotes an anti-neuroinflammatory effect and functional recovery after spinal cord injury in rats. Cell Prolif 54:e13050 (2021). PubMed: 33960559
- Hashimoto T et al. Mechanisms of Itch in Stasis Dermatitis: Significant Role of IL-31 from Macrophages. J Invest Dermatol 140:850-859.e3 (2020). PubMed: 31626785
- Kim SJ et al. Anti-inflammatory effect of Tauroursodeoxycholic acid in RAW 264.7 macrophages, Bone marrow-derived macrophages, BV2 microglial cells, and spinal cord injury. Sci Rep 8:3176 (2018). PubMed: 29453346