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Signal Transduction Metabolism Amino Acids
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Anti-Liver Arginase antibody [ARG1/1125] - BSA and Azide free (ab212522)

  • Datasheet
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Protein Array - Anti-Arginase antibody [ARG1/1125] - BSA and Azide free (ab212522)
  • Western blot - Anti-Arginase antibody [ARG1/1125] - BSA and Azide free (ab212522)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arginase antibody [ARG1/1125] - BSA and Azide free (ab212522)

Key features and details

  • Mouse monoclonal [ARG1/1125] to Liver Arginase - BSA and Azide free
  • Suitable for: Protein Array, WB, IHC-P
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG3

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Overview

  • Product name

    Anti-Liver Arginase antibody [ARG1/1125] - BSA and Azide free
    See all Liver Arginase primary antibodies
  • Description

    Mouse monoclonal [ARG1/1125] to Liver Arginase - BSA and Azide free
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Recombinant fragment
    See all applications and species data
  • Immunogen

    Recombinant fragment within Human Arginase aa 1-150. The exact sequence is proprietary.
    Database link: P05089

  • Positive control

    • Hepatocellular carcinoma tissue; Human liver tissue lysate; 293T cells

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purified from bioreactor concentrate.
  • Clonality

    Monoclonal
  • Clone number

    ARG1/1125
  • Isotype

    IgG3
  • Light chain type

    kappa
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Cancer
    • Tumor biomarkers
    • Enzymes
    • Other
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism

Associated products

  • Assay kits

    • Arginase Activity Assay Kit (Colorimetric) (ab180877)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG3, kappa monoclonal [MG3-35] - Isotype Control (ab18394)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab212522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Recombinant fragment
Application Abreviews Notes
Protein Array
Use at an assay dependent concentration.
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 35-38 kDa (predicted molecular weight: 35 kDa).
IHC-P
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
Protein Array
Use at an assay dependent concentration.
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 35-38 kDa (predicted molecular weight: 35 kDa).
IHC-P
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Pathway

    Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.
  • Involvement in disease

    Defects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia.
  • Sequence similarities

    Belongs to the arginase family.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P05089 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 383 Human
    • Omim: 608313 Human
    • SwissProt: P05089 Human
    • Unigene: 440934 Human
    • Alternative names

      • A I antibody
      • Al antibody
      • ARG 1 antibody
      • arg1 antibody
      • ARGI1_HUMAN antibody
      • Arginase 1 antibody
      • Arginase liver antibody
      • Arginase type I antibody
      • Arginase, liver antibody
      • Arginase-1 antibody
      • Arginase1 antibody
      • Liver type arginase antibody
      • Liver-type arginase antibody
      • Type I arginase antibody
      see all

    Images

    • Protein Array - Anti-Arginase antibody [ARG1/1125] - BSA and Azide free (ab212522)
      Protein Array - Anti-Arginase antibody [ARG1/1125] - BSA and Azide free (ab212522)
      This data was produced with ab215843, the same antibody in a different formulation with BSA and Azide.
      ab215843 was tested in protein array against over 19000 different full-length human proteins.
      Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
      A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
    • Western blot - Anti-Arginase antibody [ARG1/1125] - BSA and Azide free (ab212522)
      Western blot - Anti-Arginase antibody [ARG1/1125] - BSA and Azide free (ab212522)
      All lanes : Anti-Liver Arginase antibody [ARG1/1125] - BSA and Azide free (ab212522) at 0.5 µg/ml

      Lane 1 : Recombinant Arginase protein fragment
      Lane 2 : Human liver tissue lysate

      Predicted band size: 35 kDa
      Observed band size: 35-38 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arginase antibody [ARG1/1125] - BSA and Azide free (ab212522)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arginase antibody [ARG1/1125] - BSA and Azide free (ab212522)

      Immunohistochemical analysis of paraffin embedded Human hepatocellular carcinoma tissue labeling Arginase with ab212522 at 2 µg/mL.

    Protocols

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (2)

    Publishing research using ab212522? Please let us know so that we can cite the reference in this datasheet.

    ab212522 has been referenced in 2 publications.

    • Sun G  et al. Molybdenum disulfide nanoflowers mediated anti-inflammation macrophage modulation for spinal cord injury treatment. J Colloid Interface Sci 549:50-62 (2019). PubMed: 31015056
    • Tan Z  et al. Identification and characterization of microglia/macrophages in the granuloma microenvironment of encephalic schistosomiasis japonicum. BMC Infect Dis 19:1088 (2019). PubMed: 31888505

    Customer reviews and Q&As

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