Anti-Liver Arginase antibody [ARG1/1125] - BSA and Azide free (ab212522)

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Protein Array - Anti-Arginase antibody [ARG1/1125] - BSA and Azide free (ab212522)
  • Western blot - Anti-Arginase antibody [ARG1/1125] - BSA and Azide free (ab212522)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arginase antibody [ARG1/1125] - BSA and Azide free (ab212522)

Key features and details

  • Mouse monoclonal [ARG1/1125] to Liver Arginase - BSA and Azide free
  • Suitable for: Protein Array, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG3

Overview

  • Product name

    Anti-Liver Arginase antibody [ARG1/1125] - BSA and Azide free
    See all Liver Arginase primary antibodies

  • Description

    Mouse monoclonal [ARG1/1125] to Liver Arginase - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: Protein Array, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human Arginase aa 1-150. The exact sequence is proprietary.
    Database link: P05089

  • Positive control

    • Hepatocellular carcinoma tissue; Human liver tissue lysate; 293T cells

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab212522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Protein Array Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 35-38 kDa (predicted molecular weight: 35 kDa).
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Pathway

    Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.

  • Involvement in disease

    Defects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia.

  • Sequence similarities

    Belongs to the arginase family.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • A I antibody
      • Al antibody
      • ARG 1 antibody
      • arg1 antibody
      • ARGI1_HUMAN antibody
      • Arginase 1 antibody
      • Arginase liver antibody
      • Arginase type I antibody
      • Arginase, liver antibody
      • Arginase-1 antibody
      • Arginase1 antibody
      • Liver type arginase antibody
      • Liver-type arginase antibody
      • Type I arginase antibody
      see all

    Images

    • Protein Array - Anti-Arginase antibody [ARG1/1125] - BSA and Azide free (ab212522)
      Protein Array - Anti-Arginase antibody [ARG1/1125] - BSA and Azide free (ab212522)
      This data was produced with ab215843, the same antibody in a different formulation with BSA and Azide.
      ab215843 was tested in protein array against over 19000 different full-length human proteins.
      Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
      A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
    • Western blot - Anti-Arginase antibody [ARG1/1125] - BSA and Azide free (ab212522)
      Western blot - Anti-Arginase antibody [ARG1/1125] - BSA and Azide free (ab212522)
      All lanes : Anti-Liver Arginase antibody [ARG1/1125] - BSA and Azide free (ab212522) at 0.5 µg/ml

      Lane 1 : Recombinant Arginase protein fragment
      Lane 2 : Human liver tissue lysate

      Predicted band size: 35 kDa
      Observed band size: 35-38 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arginase antibody [ARG1/1125] - BSA and Azide free (ab212522)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arginase antibody [ARG1/1125] - BSA and Azide free (ab212522)

      Immunohistochemical analysis of paraffin embedded Human hepatocellular carcinoma tissue labeling Arginase with ab212522 at 2 µg/mL.

    References (2)

    Publishing research using ab212522? Please let us know so that we can cite the reference in this datasheet.

    ab212522 has been referenced in 2 publications.

    • Sun G  et al. Molybdenum disulfide nanoflowers mediated anti-inflammation macrophage modulation for spinal cord injury treatment. J Colloid Interface Sci 549:50-62 (2019). PubMed: 31015056
    • Tan Z  et al. Identification and characterization of microglia/macrophages in the granuloma microenvironment of encephalic schistosomiasis japonicum. BMC Infect Dis 19:1088 (2019). PubMed: 31888505

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