Key features and details
- Mouse monoclonal [ARG1/1125] to Liver Arginase - BSA and Azide free
- Suitable for: Protein Array, WB, IHC-P
- Reacts with: Human
- Isotype: IgG3
Product nameAnti-Liver Arginase antibody [ARG1/1125] - BSA and Azide free
See all Liver Arginase primary antibodies
DescriptionMouse monoclonal [ARG1/1125] to Liver Arginase - BSA and Azide free
Tested applicationsSuitable for: Protein Array, WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment within Human Arginase aa 1-150. The exact sequence is proprietary.
Database link: P05089
- Hepatocellular carcinoma tissue; Human liver tissue lysate; 293T cells
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Purification notesPurified from bioreactor concentrate.
Light chain typekappa
Our Abpromise guarantee covers the use of ab212522 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Protein Array||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 35-38 kDa (predicted molecular weight: 35 kDa).|
|IHC-P||Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
PathwayNitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.
Involvement in diseaseDefects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia.
Sequence similaritiesBelongs to the arginase family.
- Information by UniProt
- A I antibody
- Al antibody
- ARG 1 antibody
This data was produced with ab215843, the same antibody in a different formulation with BSA and Azide.
ab215843 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
All lanes : Anti-Liver Arginase antibody [ARG1/1125] - BSA and Azide free (ab212522) at 0.5 µg/ml
Lane 1 : Recombinant Arginase protein fragment
Lane 2 : Human liver tissue lysate
Predicted band size: 35 kDa
Observed band size: 35-38 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin embedded Human hepatocellular carcinoma tissue labeling Arginase with ab212522 at 2 µg/mL.
ab212522 has been referenced in 2 publications.
- Sun G et al. Molybdenum disulfide nanoflowers mediated anti-inflammation macrophage modulation for spinal cord injury treatment. J Colloid Interface Sci 549:50-62 (2019). PubMed: 31015056
- Tan Z et al. Identification and characterization of microglia/macrophages in the granuloma microenvironment of encephalic schistosomiasis japonicum. BMC Infect Dis 19:1088 (2019). PubMed: 31888505