Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR036F] to Liver Arginase
- Suitable for: WB, ICC, IHC-P, IP
- Reacts with: Human
Product nameAnti-Liver Arginase antibody [BLR036F]
See all Liver Arginase primary antibodies
DescriptionRabbit monoclonal [BLR036F] to Liver Arginase
Tested applicationsSuitable for: WB, ICC, IHC-P, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Liver Arginase aa 280-322 (C terminal). The exact sequence is proprietary. NP_000036.2 and Gene ID 383.
Database link: P05089
This product is sold under License from Bethyl Laboratories, Inc.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 8.20
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notesRecombinant antibody was purified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab243892 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||1/100 - 1/500.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||Use at an assay dependent concentration.
Use 20µl/mg lysate.
PathwayNitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.
Involvement in diseaseDefects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia.
Sequence similaritiesBelongs to the arginase family.
- Information by UniProt
- A I antibody
- Al antibody
- ARG 1 antibody
Liver Arginase was immunoprecipitated from 1.0 mg HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate with ab243892 at 20 µl per reaction. Western blot was performed on the immunoprecipitate using ab243892 at 1/1000 dilution.
Lane 1: ab243892 IP in HepG2 whole cell lysate.
Lane 2: Control IgG in HepG2 whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Western blot analysis using ab243892 at 1/1000 dilution.
Lane 1: HeLa whole cell lysate (50 µg).
Lane 2: HEK-293T whole cell lysate (50 µg).
Lane 3: MCF7 whole cell lysate (50 µg).
Lane 4: HepG2 whole cell lysate (50 µg).
Lane 5: A549 whole cell lysate (50 µg).
Lane 6: SW 620 whole cell lysate (50 µg).
Lane 7: Jurkat whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 30 seconds.
Formalin-fixed, paraffin-embedded HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Liver Arginase using ab243892 at 1/250 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
Formalin-fixed, paraffin-embedded human skin tissue stained for Liver Arginase using ab243892 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
Formalin-fixed, paraffin-embedded human liver carcinoma tissue stained for Liver Arginase using ab243892 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab243892 has not yet been referenced specifically in any publications.