Recombinant
RabMAb

Recombinant Anti-Liver Arginase antibody [EPR22033-369] (ab233548)

Overview

  • Product name

    Anti-Liver Arginase antibody [EPR22033-369]
    See all Liver Arginase primary antibodies
  • Description

    Rabbit monoclonal [EPR22033-369] to Liver Arginase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-P, IPmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Mouse Liver Arginase aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q61176

  • Positive control

    • WB: Mouse liver, Mouse kidney, Rat liver and Rat kidney lysates. IHC-P: Mouse liver and Rat liver tissues. FC: Hepa1-6 cells. IP: Mouse liver tissue lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab233548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/60.

 

 

WB 1/5000. Predicted molecular weight: 35 kDa.

 

 

IHC-P 1/2000.

 

 

IP 1/30.

 

 

  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Pathway

      Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.
    • Involvement in disease

      Defects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia.
    • Sequence similarities

      Belongs to the arginase family.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • A I antibody
      • Al antibody
      • ARG 1 antibody
      • arg1 antibody
      • ARGI1_HUMAN antibody
      • Arginase 1 antibody
      • Arginase liver antibody
      • Arginase type I antibody
      • Arginase, liver antibody
      • Arginase-1 antibody
      • Arginase1 antibody
      • Liver type arginase antibody
      • Liver-type arginase antibody
      • Type I arginase antibody
      see all

    Images

    • All lanes : Anti-Liver Arginase antibody [EPR22033-369] (ab233548) at 1/5000 dilution

      Lane 1 : Mouse liver lysate
      Lane 2 : Mouse kidney lysate
      Lane 3 : Rat liver lysate
      Lane 4 : Rat kidney lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
      Lanes 3-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 35 kDa
      Observed band size: 37 kDa
      why is the actual band size different from the predicted?



      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 27761413).   Negative control: kidney (PMID: 21975054)

      Blocking/Diluting buffer and concentration: 5% NFDM/TBST 

      Exposure Time: 1 second

    • Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Liver Arginase with ab233548 at 1/2000 dilution (0.333 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear and cytoplasmic staining in rat liver (PMID/ 22298472, 23637937, 21975054) is observed. The section was incubated with ab233548 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Liver Arginase with ab233548 at 1/2000 dilution (0.333 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear and cytoplasmic staining in mouse liver (PMID/ 22298472, 23637937, 21975054). The section was incubated with ab233548 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Liver Arginase was immunoprecipitated from 0.35 mg Mouse liver tissue lysate with ab233548 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab233548 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

      Lane 1: Mouse liver tissue lysate 10ug

      Lane 2: ab233548 IP in Mouse liver tissue lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab233548 in Mouse liver tissue lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST

      Exposure time: 3 seconds

       

    • Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Hepa1-6 (mouse epithelial hepatoma) cells labeling Liver Arginase with ab233548 at 1/60 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    References

    ab233548 has not yet been referenced specifically in any publications.

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