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RecombinantRabMAb

Recombinant Anti-Liver Arginase antibody [EPR6671(B)] (ab124917)

  • Datasheet
Reviews (1)Q&A (1)References (14)

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Western blot - Anti-Liver Arginase antibody [EPR6671(B)] (ab124917)
  • Anti-Liver Arginase antibody [EPR6671(B)] (ab124917)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6671(B)] to Liver Arginase
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Liver Arginase antibody [EPR6671(B)]
    See all Liver Arginase primary antibodies
  • Description

    Rabbit monoclonal [EPR6671(B)] to Liver Arginase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Liver Arginase aa 1-100. The exact sequence is proprietary.
    Database link: P05089

  • Positive control

    • WB: Human fetal liver and mouse liver lysates.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR6671(B)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Alternative Versions

    • Anti-Liver Arginase antibody [EPR6671(B)] - BSA and Azide free (ab248058)
  • Assay kits

    • Arginase Activity Assay Kit (Colorimetric) (ab180877)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Mouse liver tissue lysate - total protein (ab29301)
  • Recombinant Protein

    • Recombinant Human Liver Arginase protein (ab95487)

Applications

Our Abpromise guarantee covers the use of ab124917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Pathway

      Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.
    • Involvement in disease

      Defects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia.
    • Sequence similarities

      Belongs to the arginase family.
    • Cellular localization

      Cytoplasm.
    • Target information above from: UniProt accession P05089 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 383 Human
      • Entrez Gene: 11846 Mouse
      • Entrez Gene: 29221 Rat
      • Omim: 608313 Human
      • SwissProt: P05089 Human
      • SwissProt: Q61176 Mouse
      • SwissProt: P07824 Rat
      • Unigene: 440934 Human
      • Unigene: 154144 Mouse
      • Unigene: 9857 Rat
      see all
    • Alternative names

      • A I antibody
      • Al antibody
      • ARG 1 antibody
      • arg1 antibody
      • ARGI1_HUMAN antibody
      • Arginase 1 antibody
      • Arginase liver antibody
      • Arginase type I antibody
      • Arginase, liver antibody
      • Arginase-1 antibody
      • Arginase1 antibody
      • Liver type arginase antibody
      • Liver-type arginase antibody
      • Type I arginase antibody
      see all

    Images

    • Western blot - Anti-Liver Arginase antibody [EPR6671(B)] (ab124917)
      Western blot - Anti-Liver Arginase antibody [EPR6671(B)] (ab124917)
      All lanes : Anti-Liver Arginase antibody [EPR6671(B)] (ab124917) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : Mouse liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 35 kDa

    • Anti-Liver Arginase antibody [EPR6671(B)] (ab124917)
      Anti-Liver Arginase antibody [EPR6671(B)] (ab124917)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (14)

    Publishing research using ab124917? Please let us know so that we can cite the reference in this datasheet.

    ab124917 has been referenced in 14 publications.

    • Li Y  et al. Bone marrow macrophage M2 polarization and adipose-derived stem cells osteogenic differentiation synergistically promote rehabilitation of bone damage. J Cell Biochem 120:19891-19901 (2019). PubMed: 31338874
    • Chang JC  et al. Adaptive adipose tissue stromal plasticity in response to cold stress and antibody-based metabolic therapy. Sci Rep 9:8833 (2019). IHC-P . PubMed: 31222070
    • Yuan C  et al. OAB-14, a bexarotene derivative, improves Alzheimer's disease-related pathologies and cognitive impairments by increasing ß-amyloid clearance in APP/PS1 mice. Biochim Biophys Acta Mol Basis Dis 1865:161-180 (2019). WB . PubMed: 30389579
    • Miller CN  et al. Guggulsterone Activates Adipocyte Beiging through Direct Effects on 3T3-L1 Adipocytes and Indirect Effects Mediated through RAW264.7 Macrophages. Medicines (Basel) 6:N/A (2019). WB . PubMed: 30709026
    • Zhao N  et al. Lipocalin-2 may produce damaging effect after cerebral ischemia by inducing astrocytes classical activation. J Neuroinflammation 16:168 (2019). PubMed: 31426811
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-Liver Arginase antibody [EPR6671(B)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Bone Marrow-Derived Macrophages)
    Gel Running Conditions
    Reduced Denaturing (10% polyacrylamide gel)
    Loading amount
    160000 cells
    Treatment
    20 ng/mL mouse IL-4 for 24 hours
    Specification
    Bone Marrow-Derived Macrophages
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    祐介 安藤

    Verified customer

    Submitted Nov 16 2016

    Question

    WB with human and mouse cell line
    sees bands also in neg. crtl (macrophages)
    cells need to be activated to express liver arginase
    cell lysate: 30 kDa consistantly, also in neg. crtl
    homogenate (mouse liver and lung): 35 kDa
    Ab: 1/2000 o/n
    block: 5% milk
    2nd: works well

    Read More

    Abcam community

    Verified customer

    Asked on Jan 24 2013

    Answer

    we have issued a free of charge replacement

    Read More

    Abcam Scientific Support

    Answered on Jan 24 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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