Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6672(B)] to Liver Arginase - BSA and Azide free
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
Product nameAnti-Liver Arginase antibody [EPR6672(B)] - BSA and Azide free
See all Liver Arginase primary antibodies
DescriptionRabbit monoclonal [EPR6672(B)] to Liver Arginase - BSA and Azide free
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Liver Arginase aa 300-400. The exact sequence is proprietary.
- Human fetal liver and Human fetal lung lysates; Human hepatocellular carcinoma tissue.
Ab211961 is the carrier-free version of ab133543. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab211961 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab211961 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).|
|IP||Use at an assay dependent concentration.|
PathwayNitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.
Involvement in diseaseDefects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia.
Sequence similaritiesBelongs to the arginase family.
- Information by UniProt
- A I antibody
- Al antibody
- ARG 1 antibody
Lane 1 (input): Human fetal liver whole cell lysate, 10μg
Lane 2: Human fetal liver whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab133543 in Human fetal liver whole cell lysate
Ab133543 immunoprecipitating liver arginase in Human fetal liver whole cell lysates. Primary antibody was used at a 1:1000 dilution (0.27 μg/ml). For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution. Capture antibody was used at 1:20 dilution (1.3μg in 0.35mg lysates).
Blocking and diluting buffer used: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133543).
This IHC data was generated using the same anti-Liver Arginase antibody clone, EPR6672(B), in a different buffer formulation (cat# ab133543).
Immunohistochemical analysis of paraffin embedded Human hepatocellular carcinoma tissue labelling liver Arginase with ab133543 antibody at a dilution of 1/250.
ab211961 has been referenced in 1 publication.
- Ordóñez NG Arginase-1 is a novel immunohistochemical marker of hepatocellular differentiation. Adv Anat Pathol 21:285-90 (2014). PubMed: 24911253