Recombinant
RabMAb

Recombinant Anti-Liver Arginase antibody [EPR6672(B)] - BSA and Azide free (ab211961)

Overview

  • Product name

    Anti-Liver Arginase antibody [EPR6672(B)] - BSA and Azide free
    See all Liver Arginase primary antibodies
  • Description

    Rabbit monoclonal [EPR6672(B)] to Liver Arginase - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Liver Arginase aa 300-400. The exact sequence is proprietary.

  • Positive control

    • Human fetal liver and Human fetal lung lysates; Human hepatocellular carcinoma tissue.
  • General notes

    Ab211961 is the carrier-free version of ab133543. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab211961 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab211961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
IP Use at an assay dependent concentration.

Target

  • Pathway

    Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.
  • Involvement in disease

    Defects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia.
  • Sequence similarities

    Belongs to the arginase family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • A I antibody
    • Al antibody
    • ARG 1 antibody
    • arg1 antibody
    • ARGI1_HUMAN antibody
    • Arginase 1 antibody
    • Arginase liver antibody
    • Arginase type I antibody
    • Arginase, liver antibody
    • Arginase-1 antibody
    • Arginase1 antibody
    • Liver type arginase antibody
    • Liver-type arginase antibody
    • Type I arginase antibody
    see all

Images

  • Lane 1 (input): Human fetal liver whole cell lysate, 10μg
    Lane 2: Human fetal liver whole cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab133543 in Human fetal liver whole cell lysate

    Ab133543 immunoprecipitating liver arginase in Human fetal liver whole cell lysates. Primary antibody was used at a 1:1000 dilution (0.27 μg/ml). For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.  Capture antibody was used at 1:20 dilution (1.3μg in 0.35mg lysates).

    Blocking and diluting buffer used: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133543).

  • This IHC data was generated using the same anti-Liver Arginase antibody clone, EPR6672(B), in a different buffer formulation (cat# ab133543).

    Immunohistochemical analysis of paraffin embedded Human hepatocellular carcinoma tissue labelling liver Arginase with ab133543 antibody at a dilution of 1/250.

References

This product has been referenced in:

See 1 Publication for this product

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