Recombinant
RabMAb

Recombinant Anti-Liver Arginase antibody [SP156] (ab183333)

Overview

  • Product name

    Anti-Liver Arginase antibody [SP156]
    See all Liver Arginase primary antibodies
  • Description

    Rabbit monoclonal [SP156] to Liver Arginase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Cow, Pig
  • Immunogen

    Synthetic peptide within Human Liver Arginase aa 300 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P05089

  • Positive control

    • IHC-P: Human liver, hepatocellular carcinoma, and liver cirrhosis tissue.
  • General notes

     

     

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab183333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.

Boil tissue section in 1mM EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.

Target

  • Pathway

    Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.
  • Involvement in disease

    Defects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia.
  • Sequence similarities

    Belongs to the arginase family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • A I antibody
    • Al antibody
    • ARG 1 antibody
    • arg1 antibody
    • ARGI1_HUMAN antibody
    • Arginase 1 antibody
    • Arginase liver antibody
    • Arginase type I antibody
    • Arginase, liver antibody
    • Arginase-1 antibody
    • Arginase1 antibody
    • Liver type arginase antibody
    • Liver-type arginase antibody
    • Type I arginase antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocyte cancer tissue sections labeling Liver Arginase with Purified ab183333 at 1/100 dilution (0.54 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Liver Arginase with ab183333 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human liver cirrhosis tissue labeling Liver Arginase with ab183333 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human liver cirrhosis tissue labeling Liver Arginase with ab183333 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.

References

ab183333 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cow Tissue sections (Lung and Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
Lung and Liver
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 25% · Temperature: 22°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jun 13 2016

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