Recombinant Anti-Liver Arginase antibody [SP156] (ab183333)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP156] to Liver Arginase
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Liver Arginase antibody [SP156]
See all Liver Arginase primary antibodies -
Description
Rabbit monoclonal [SP156] to Liver Arginase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Cow, Pig -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human liver, hepatocellular carcinoma, and liver cirrhosis tissue; WB: Mouse liver and human liver.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP156 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183333 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1.86 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa).
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IHC-P |
1/100.
Boil tissue section in 1mM EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min. |
Notes |
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WB
Use a concentration of 1.86 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa). |
IHC-P
1/100. Boil tissue section in 1mM EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min. |
Target
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Pathway
Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1. -
Involvement in disease
Defects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia. -
Sequence similarities
Belongs to the arginase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 383 Human
- Entrez Gene: 11846 Mouse
- Omim: 608313 Human
- SwissProt: P05089 Human
- SwissProt: Q61176 Mouse
- Unigene: 440934 Human
- Unigene: 154144 Mouse
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Alternative names
- A I antibody
- Al antibody
- ARG 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocyte cancer tissue sections labeling Liver Arginase with Purified ab183333 at 1/100 dilution (0.54 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-Liver Arginase antibody [SP156] (ab183333) at 1.866 µg/ml
Lane 1 : Mouse liver tissue
Lane 2 : Mouse spleen tissue
Lane 3 : Human liver tissue
Lane 4 : Human spleen tissue
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Predicted band size: 35 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?Exposure time:
Lane1 & 2 is 3min
Lane 3 & 4 is 4 s -
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Liver Arginase with ab183333 at 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human liver cirrhosis tissue labeling Liver Arginase with ab183333 at 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human liver cirrhosis tissue labeling Liver Arginase with ab183333 at 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab183333 has been referenced in 4 publications.
- Stalder R et al. Interleukin-17E, inducible nitric oxide synthase and arginase1 as new biomarkers in the identification of neutrophilic dermatoses. Clin Exp Dermatol 47:675-683 (2022). PubMed: 34669971
- Zhu Y et al. Flavokawain B Weakens Gastric Cancer Progression via the TGF-β1/SMAD4 Pathway and Attenuates M2 Macrophage Polarization. J Immunol Res 2022:4903333 (2022). PubMed: 35879950
- Stalder R et al. The Janus Kinase inhibitor tofacitinib impacts human dendritic cell differentiation and favours M1 macrophage development. Exp Dermatol 29:71-78 (2020). PubMed: 31721311
- Patil P et al. Porcine Ischemic Wound-Healing Model for Preclinical Testing of Degradable Biomaterials. Tissue Eng Part C Methods 23:754-762 (2017). PubMed: 28762881