Recombinant
RabMAb

Recombinant Anti-Liver Arginase antibody [SP156] - BSA and Azide free (ab236233)

Overview

  • Product name

    Anti-Liver Arginase antibody [SP156] - BSA and Azide free
    See all Liver Arginase primary antibodies
  • Description

    Rabbit monoclonal [SP156] to Liver Arginase - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Cow, Pig
  • Immunogen

    Synthetic peptide within Human Liver Arginase (C terminal). The exact sequence is proprietary.
    Database link: P05089

  • Positive control

    • IHC-P: Human liver, hepatocellular carcinoma, and liver cirrhosis tissue.
  • General notes

    Ab236233 is the carrier-free version of ab183333. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab236233 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab236233 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

Boil tissue section in 1mM EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.

Target

  • Pathway

    Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.
  • Involvement in disease

    Defects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia.
  • Sequence similarities

    Belongs to the arginase family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • A I antibody
    • Al antibody
    • ARG 1 antibody
    • arg1 antibody
    • ARGI1_HUMAN antibody
    • Arginase 1 antibody
    • Arginase liver antibody
    • Arginase type I antibody
    • Arginase, liver antibody
    • Arginase-1 antibody
    • Arginase1 antibody
    • Liver type arginase antibody
    • Liver-type arginase antibody
    • Type I arginase antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocyte cancer tissue sections labeling Liver Arginase with Purified ab183333 at 1/100 dilution (0.54 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183333)
  • Formalin-fixed, paraffin-embedded human hepatocellular carcinoma tissue stained for Liver Arginase using ab228264 in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228264).

  • Formalin-fixed, paraffin-embedded human hepatocellular carcinoma tissue stained for Liver Arginase using ab228264 in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228264).

  • Formalin-fixed, paraffin-embedded human hepatocellular carcinoma tissue stained for Liver Arginase using ab228264 in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228264).

  • Formalin-fixed, paraffin-embedded human liver tissue stained for Liver Arginase using ab228264 in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228264).

  • Formalin-fixed, paraffin-embedded human liver cirrhosis tissue stained for Liver Arginase using ab228264 in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228264).

  • Formalin-fixed, paraffin-embedded human hepatocellular carcinoma tissue stained for Liver Arginase using ab228264 in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228264).

  • Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling Liver Arginase with ab183333 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183333).

  • Formalin-fixed, paraffin-embedded human liver cirrhosis tissue stained for Liver Arginase using ab228264 in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228264).

  • Formalin-fixed, paraffin-embedded human hepatocellular carcinoma tissue stained for Liver Arginase using ab228264 in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228264).

  • Formalin-fixed, paraffin-embedded human hepatocellular carcinoma tissue stained for Liver Arginase using ab228264 in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228264).

  • Formalin-fixed, paraffin-embedded human hepatocellular carcinoma tissue stained for Liver Arginase using ab228264 in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228264).

References

ab236233 has not yet been referenced specifically in any publications.

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