Product nameLiver Arginase overexpression 293T lysate (whole cell)
See all Liver Arginase lysates
ab94083 is a 293T cell transfected lysate in which Human liver Arginase has been transiently over-expressed using a pCMV-ARG1 Full length plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes.
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
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BackgroundPathway: Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1. Disease: Defects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia. Similarity: Belongs to the arginase family.
Our Abpromise guarantee covers the use of ab94083 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94083 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-liver Arginase antibody (ab89184) at 1/500 dilution
Lane 1 :
Liver Arginase overexpression 293T lysate (whole cell) (ab94083)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94083 has not yet been referenced specifically in any publications.