Anti-Liver Carboxylesterase 1/CES1 antibody (ab115280)
Key features and details
- Goat polyclonal to Liver Carboxylesterase 1/CES1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Liver Carboxylesterase 1/CES1 antibody
See all Liver Carboxylesterase 1/CES1 primary antibodies -
Description
Goat polyclonal to Liver Carboxylesterase 1/CES1 -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Macaque monkey, Orangutan -
Immunogen
Synthetic peptide corresponding to Human Liver Carboxylesterase 1/CES1 aa 500-600 (internal sequence).
Database link: P23141 -
Positive control
- Recombinant Human Liver Carboxylesterase 1/CES1 protein (ab114347) can be used as a positive control in WB. Human lung tissue. Human liver lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Constituents: 99% Tris buffered saline, 0.02% Sodium chloride, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab115280 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 63 kDa.
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IHC-P |
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 63 kDa. |
IHC-P
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate. -
Tissue specificity
Expressed predominantly in liver with lower levels in heart and lung. -
Sequence similarities
Belongs to the type-B carboxylesterase/lipase family. -
Post-translational
modificationsContains sialic acid.
Cleavage of the signal sequence can occur at 2 positions, either between Trp-17 and Gly-18 or between Gly-18 and His-19. -
Cellular localization
Endoplasmic reticulum lumen. - Information by UniProt
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Database links
- Entrez Gene: 1066 Human
- Omim: 114835 Human
- SwissProt: P23141 Human
- Unigene: 558865 Human
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Alternative names
- ACAT antibody
- Acyl coenzyme A cholesterol acyltransferase antibody
- Acyl-coenzyme A:cholesterol acyltransferase antibody
see all
Images
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Human Lung formalin-fixed, paraffin-embedded tissue stained with ab115280 at 2.5 ug/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Anti-Liver Carboxylesterase 1/CES1 antibody (ab115280) at 0.03 µg/ml + Human Liver lysate in RIPA buffer (incubation time: 1 hour) at 35 µg
Developed using the ECL technique.
Predicted band size: 63 kDa
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab115280 has not yet been referenced specifically in any publications.