Liver Carboxylesterase 1 overexpression 293T lysate (whole cell) (ab94047)
Overview
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Product name
Liver Carboxylesterase 1 overexpression 293T lysate (whole cell)
See all Liver Carboxylesterase 1/CES1 lysates -
General notes
ab94047 is a 293T cell transfected lysate in which Human Liver Carboxylesterase 1 has been transiently over-expressed using a pCMV-Liver Carboxylesterase 1 plasmid. The lysate is provided in 1X Sample Buffer.
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Research areas
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Background
Function: Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate. PTM: Contains sialic acid. Cleavage of the signal sequence can occur at 2 positions, either between Trp-17 and Gly-18 or between Gly-18 and His-19. Similarity: Belongs to the type-B carboxylesterase/lipase family. Tissue specificity: Expressed predominantly in liver with lower levels in heart and lung.
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab94047 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent dilution.
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Notes |
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WB
Use at an assay dependent dilution. |
Images
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ab94047 at 15µg/lane on an SDS-PAGE gel.
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All lanes : Anti-Liver Carboxylesterase 1/CES1 antibody (ab89779) at 1/500 dilution
Lane 1 :Liver Carboxylesterase 1 overexpression 293T lysate (whole cell) (ab94047)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab94047 has not yet been referenced specifically in any publications.