Anti-Liver Carboxylesterase 1/CES1 antibody - C-terminal (ab180705)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Liver Carboxylesterase 1/CES1 antibody - C-terminal
See all Liver Carboxylesterase 1/CES1 primary antibodies -
Description
Rabbit polyclonal to Liver Carboxylesterase 1/CES1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Recombinant fragment within Human Liver Carboxylesterase 1/CES1 aa 287-567 (C terminal). The exact sequence is proprietary.
Sequence:RQKT EEELLETTLK MKFLSLDLQG DPRESQPLLG TVIDGMLLLK TPEELQAERN FHTVPYMVGI NKQEFGWLIP MQLMSYPLSE GQLDQKTAMS LLWKSYPLVC IAKELIPEAT EKYLGGTDDT VKKKDLFLDL IADVMFGVPS VIVARNHRDA GAPTYMYEFQ YRPSFSSDMK PKTVIGDHGD ELFSVFGAPF LKEGASEEEI RLSKMVMKFW ANFARNGNPN GEGLPHWPEY NQKEGYLQIG ANTQAAQKLK DKEVAFWTNL FAKKAVEKPP QTEHIEL
Database link: P23141 -
Positive control
- Mouse liver, A549 and U937 cell extracts.
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General notes
This product was previously labelled as Liver Carboxylesterase 1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab180705 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/2000. Predicted molecular weight: 62 kDa. | |
| IHC-P | 1/50 - 1/200. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate. -
Tissue specificity
Expressed predominantly in liver with lower levels in heart and lung. -
Sequence similarities
Belongs to the type-B carboxylesterase/lipase family. -
Post-translational
modificationsContains sialic acid.
Cleavage of the signal sequence can occur at 2 positions, either between Trp-17 and Gly-18 or between Gly-18 and His-19. -
Cellular localization
Endoplasmic reticulum lumen. - Information by UniProt
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Database links
- Entrez Gene: 1066 Human
- Entrez Gene: 12623 Mouse
- Entrez Gene: 24346 Rat
- Entrez Gene: 29225 Rat
- Omim: 114835 Human
- SwissProt: P23141 Human
- SwissProt: Q8VCC2 Mouse
- SwissProt: P10959 Rat
see all -
Alternative names
- ACAT antibody
- Acyl coenzyme A cholesterol acyltransferase antibody
- Acyl-coenzyme A:cholesterol acyltransferase antibody
see all
Images
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Western blot - Anti-Liver Carboxylesterase 1/CES1 antibody - C-terminal (ab180705)All lanes : Anti-Liver Carboxylesterase 1/CES1 antibody - C-terminal (ab180705) at 1/500 dilution
Lane 1 : Mouse liver cell extract
Lane 2 : A549 cell extract
Lane 3 : U937 cell extract
Predicted band size: 62 kDa
Datasheets and documents
References
ab180705 has not yet been referenced specifically in any publications.
