• Product name

    Anti-Liver Carboxylesterase 1/CES1 antibody [EP1376Y]
    See all Liver Carboxylesterase 1/CES1 primary antibodies
  • Description

    Rabbit monoclonal [EP1376Y] to Liver Carboxylesterase 1/CES1
  • Host species

  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Liver Carboxylesterase 1/CES1 aa 550 to the C-terminus (C terminal). The exact sequence is proprietary.

  • Positive control

    • U937 cell lysates Human liver tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     This product was previously labelled as Liver Carboxylesterase 1


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.


Our Abpromise guarantee covers the use of ab53008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 65 kDa).
IP 1/100.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate.
    • Tissue specificity

      Expressed predominantly in liver with lower levels in heart and lung.
    • Sequence similarities

      Belongs to the type-B carboxylesterase/lipase family.
    • Post-translational

      Contains sialic acid.
      Cleavage of the signal sequence can occur at 2 positions, either between Trp-17 and Gly-18 or between Gly-18 and His-19.
    • Cellular localization

      Endoplasmic reticulum lumen.
    • Information by UniProt
    • Database links

    • Alternative names

      • ACAT antibody
      • Acyl coenzyme A cholesterol acyltransferase antibody
      • Acyl-coenzyme A:cholesterol acyltransferase antibody
      • Brain carboxylesterase hBr1 antibody
      • Carboxyesterase ES-3 antibody
      • Carboxylesterase antibody
      • Carboxylesterase 1 (monocyte/macrophage serine esterase 1) antibody
      • Carboxylesterase 1 antibody
      • Carboxylesterase 1 deficiency, included antibody
      • Carboxylesterase 2, formerly antibody
      • CE 1 antibody
      • CEH antibody
      • Ces-1 antibody
      • CES1 antibody
      • CES2 antibody
      • CESDD1 antibody
      • Cholesterol ester hydrolase, neutral, macrophage-derived antibody
      • Cholesteryl ester hydrolase antibody
      • Cocaine carboxylesterase antibody
      • EC antibody
      • Egasyn antibody
      • ES-HTEL antibody
      • ES-x antibody
      • Es22 antibody
      • EST1_HUMAN antibody
      • Esterase 22 antibody
      • Esterase antibody
      • hCE 1 antibody
      • HMSE antibody
      • HMSE1 antibody
      • Liver carboxylesterase 1 antibody
      • Liver carboxylesterase 3 antibody
      • Methylumbelliferyl acetate deacetylase 1 antibody
      • MGC117365 antibody
      • MGC156521 antibody
      • Monocyte carboxylesterase deficiency, included antibody
      • Monocyte esterase deficiency, included antibody
      • Monocyte/macrophage serine esterase antibody
      • PCE-1 antibody
      • pI 5.5 esterase antibody
      • Proline-beta-naphthylamidase antibody
      • REH antibody
      • Retinyl ester hydrolase antibody
      • Serine esterase 1 antibody
      • Ses-1 antibody
      • SES1 antibody
      • TGH antibody
      • Triacylglycerol hydrolase antibody
      see all


    • Anti-Liver Carboxylesterase 1/CES1 antibody [EP1376Y] (ab53008) at 1/10000 dilution + U937 cell lysate at 10 µg

      goat anti-rabbit HRP labelled at 1/2000 dilution

      Predicted band size: 65 kDa
      Observed band size: 60 kDa
      why is the actual band size different from the predicted?

    • ab53008, at a 1/250 dilution, staining Human SOAT 1 in Liver using Immunohistochemistry, Paraffin embedded tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


    This product has been referenced in:

    • Malty RH  et al. A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and NF-?B Signaling. Cell Syst 5:564-577.e12 (2017). Read more (PubMed: 29128334) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A


    Thank you for contacting us. These two antibodies should work equally well in IHC for Human. ab53008 has not successfully been tested for other species. They do have differenct immunogenic peptides but that doesn't mean one should work better than the other. The WB images both show a band around 50 kD. The additional band you see in ab39327 is detecting the protein in the native form. They would both be guaranteed to work in human for the species lsited, respectively.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More


    Thank you for your reply.

    Our ab39327 is actually capable of detecting both the native a processed form of the protein. I've attached the WB image and here is the caption:

    Human ACAT-1 has 550 amino acids with an estimated MW of 65 kDa (1). Abcam's affinity purified antibody recognizes a 50 kDa band in most cultured cell lines as reported in the literature (2,3,4). In addition, the antibody reacts with a native form of ACAT-1 at about 65 kDa in liver tissues.

    1) Chang, C.C.Y., Huh, H.Y., Cadigan, K.M., et al. J. Biol. Chem. 268(28), 20747-20755 (1993).
    2) Lee, R.G., Willingham, M.C., Davis, M.A., et al. J. Lipid Res. 41, 1991 - 2001 (2000).
    3) Chang, C.C.Y., Lee, C.-Y.G., Chang, E.T., et al. J. Biol. Chem. 273(52), 35132-35141 (1998).
    4) Chang, C.C.Y., Chen, J., Thomas, M.A., et al. J. Biol. Chem. 270(49), 29532-29540 (1995).

    I will have the WB image added to the datasheet.

    I hope this information helps. Please contact us with any other questions.

    Read More


    Thank you for contacting us.

    The MW of SOAT1 according to SwissProt is 64.7 kDa and it is 550 kDa long.


    There may be a processed form of the protein which other antibodies can detect, but for ab53008 we know it detects the full length form.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    For licensing inquiries, please contact partnerships@abcam.com

    Sign up