Overview

  • Product name

    Anti-Liver Carboxylesterase 1/CES1 antibody [EP1376Y] - BSA and Azide free
    See all Liver Carboxylesterase 1/CES1 primary antibodies
  • Description

    Rabbit monoclonal [EP1376Y] to Liver Carboxylesterase 1/CES1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Liver Carboxylesterase 1/CES1 aa 550 to the C-terminus (C terminal). The exact sequence is proprietary.

  • Positive control

    • IHC-P: Human liver tissue.
  • General notes

    ab235996 is the carrier-free version of ab53008 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab235996 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.  This product was previously labelled as Liver Carboxylesterase 1

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab235996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 65 kDa).

Target

  • Function

    Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate.
  • Tissue specificity

    Expressed predominantly in liver with lower levels in heart and lung.
  • Sequence similarities

    Belongs to the type-B carboxylesterase/lipase family.
  • Post-translational
    modifications

    Contains sialic acid.
    Cleavage of the signal sequence can occur at 2 positions, either between Trp-17 and Gly-18 or between Gly-18 and His-19.
  • Cellular localization

    Endoplasmic reticulum lumen.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACAT antibody
    • Acyl coenzyme A cholesterol acyltransferase antibody
    • Acyl-coenzyme A:cholesterol acyltransferase antibody
    • Brain carboxylesterase hBr1 antibody
    • Carboxyesterase ES-3 antibody
    • Carboxylesterase antibody
    • Carboxylesterase 1 (monocyte/macrophage serine esterase 1) antibody
    • Carboxylesterase 1 antibody
    • Carboxylesterase 1 deficiency, included antibody
    • Carboxylesterase 2, formerly antibody
    • CE 1 antibody
    • CEH antibody
    • Ces-1 antibody
    • CES1 antibody
    • CES2 antibody
    • CESDD1 antibody
    • Cholesterol ester hydrolase, neutral, macrophage-derived antibody
    • Cholesteryl ester hydrolase antibody
    • Cocaine carboxylesterase antibody
    • EC 3.1.1.1 antibody
    • Egasyn antibody
    • ES-HTEL antibody
    • ES-x antibody
    • Es22 antibody
    • EST1_HUMAN antibody
    • Esterase 22 antibody
    • Esterase antibody
    • hCE 1 antibody
    • HMSE antibody
    • HMSE1 antibody
    • Liver carboxylesterase 1 antibody
    • Liver carboxylesterase 3 antibody
    • Methylumbelliferyl acetate deacetylase 1 antibody
    • MGC117365 antibody
    • MGC156521 antibody
    • Monocyte carboxylesterase deficiency, included antibody
    • Monocyte esterase deficiency, included antibody
    • Monocyte/macrophage serine esterase antibody
    • PCE-1 antibody
    • pI 5.5 esterase antibody
    • Proline-beta-naphthylamidase antibody
    • REH antibody
    • Retinyl ester hydrolase antibody
    • Serine esterase 1 antibody
    • Ses-1 antibody
    • SES1 antibody
    • TGH antibody
    • Triacylglycerol hydrolase antibody
    see all

Images

  • ab53008, at a 1/250 dilution, staining Human SOAT 1 in Liver using Immunohistochemistry, Paraffin embedded tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab53008).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

References

ab235996 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab235996.
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