Overview

  • Product name

    Anti-liver FABP antibody [2G4]
    See all liver FABP primary antibodies
  • Description

    Mouse monoclonal [2G4] to liver FABP
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length Human liver FABP (aa1-127) purified from E. coli (NP_001434)

  • Positive control

    • WB: HepG2 whole cell lysate (ab7900), FABP1 Recombinant protein, Liver tissues lysate. ICC/IF: Hep3B cells. FC: Hep3B cells.
  • General notes

    Isotype: Mouse IgG1 heavy chain and kappa light chain

    This product was changed from ascites to tissue culture supernatant on 24/01/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    2G4
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

WB Use at an assay dependent concentration. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport.
  • Sequence similarities

    Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • Domain

    Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • FABP 1 antibody
    • FABP1 antibody
    • FABPL antibody
    • FABPL_HUMAN antibody
    • Fatty Acid Binding Protein 1 antibody
    • Fatty acid binding protein 1 liver antibody
    • Fatty Acid Binding Protein antibody
    • Fatty acid-binding protein 1 antibody
    • Fatty acid-binding protein antibody
    • Fatty acid-binding protein liver antibody
    • L FABP antibody
    • L-FABP antibody
    • liver antibody
    • Liver-type fatty acid-binding protein antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence analysis of FABP1 in Hep3B cells. The cell was stained with ab82157 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
  • Flow cytometry analysis of FABP1 in Hep3B cells. The cell was stained with ab82157 at 2-5µg for 1x10cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black).

  • All lanes : Anti-liver FABP antibody [2G4] (ab82157) at 1/1000 dilution

    Lane 1 : FABP1 Recombinant protein
    Lane 2 : HepG2 cell lysate at 40 µg
    Lane 3 : Liver tissues lysate at 40 µg

    Secondary
    All lanes : goat anti-mouse secondary antibody conjugated to HRP

    Predicted band size: 14 kDa

  • Anti-liver FABP antibody [2G4] (ab82157) at 1/1000 dilution + HepG2 cell lysate at 30 µg

    Secondary
    goat anti-mouse HRP

    Developed using the ECL technique.

    Predicted band size: 14 kDa
    Observed band size: 14 kDa



    This image was produced using the ascites version of this antibody. 

  • Overlay histogram showing HepG2 cells stained with ab82157 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab82157, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    This image was produced using the ascites version of this antibody. 

  • Ab82157 staining liver FABP in Hep3B cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Samples were incubated with primary antibody at 1:100 dilution. An Alexa Fluor® 488 goat anti-mouse IgG was used as the secondary antibody. DAPI was used as a nuclear counterstain.

    This image was produced using the ascites version of this antibody. 

References

This product has been referenced in:

  • Schlabritz-Loutsevitch N  et al. A first case of hepatocellular carcinoma in the baboon (Papio spp.) placenta. J Med Primatol 48:68-73 (2019). Read more (PubMed: 30246873) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for contacting us.

Unfortunately, we offer the antibody only in the size listed on the datasheet (100 ul). Sample sizes cannot be offered due to operational reasons. For larger sizes or bulk orders, please contact mailto:sales@abcam.comfor more information.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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