Anti-liver FABP antibody [2G4] (ab82157)
Key features and details
- Mouse monoclonal [2G4] to liver FABP
- Suitable for: Flow Cyt, WB, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-liver FABP antibody [2G4]
See all liver FABP primary antibodies -
Description
Mouse monoclonal [2G4] to liver FABP -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant full length Human liver FABP (aa1-127) purified from E. coli (NP_001434)
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Positive control
- WB: HepG2 whole cell lysate (ab7900), FABP1 Recombinant protein, Liver tissues lysate. ICC/IF: Hep3B cells. Flow Cyt: Hep3B cells.
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General notes
Isotype: Mouse IgG1 heavy chain and kappa light chain
This product was changed from ascites to tissue culture supernatant on 24/01/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 10% Glycerol -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
2G4 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab82157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
Use at an assay dependent concentration. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa). |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport. -
Sequence similarities
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
Domain
Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 2168 Human
- Omim: 134650 Human
- SwissProt: P07148 Human
- Unigene: 380135 Human
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Alternative names
- FABP 1 antibody
- FABP1 antibody
- FABPL antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence analysis of FABP1 in Hep3B cells. The cell was stained with ab82157 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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Flow cytometry analysis of FABP1 in Hep3B cells. The cell was stained with ab82157 at 2-5µg for 1x106 cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black).
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All lanes : Anti-liver FABP antibody [2G4] (ab82157) at 1/1000 dilution
Lane 1 : FABP1 Recombinant protein
Lane 2 : HepG2 cell lysate at 40 µg
Lane 3 : Liver tissues lysate at 40 µg
Secondary
All lanes : goat anti-mouse secondary antibody conjugated to HRP
Predicted band size: 14 kDa -
Anti-liver FABP antibody [2G4] (ab82157) at 1/1000 dilution + HepG2 cell lysate at 30 µg
Secondary
goat anti-mouse HRP
Developed using the ECL technique.
Predicted band size: 14 kDa
Observed band size: 14 kDaThis image was produced using the ascites version of this antibody.
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Overlay histogram showing HepG2 cells stained with ab82157 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab82157, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was produced using the ascites version of this antibody.
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Ab82157 staining liver FABP in Hep3B cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Samples were incubated with primary antibody at 1:100 dilution. An Alexa Fluor® 488 goat anti-mouse IgG was used as the secondary antibody. DAPI was used as a nuclear counterstain.
This image was produced using the ascites version of this antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab82157 has been referenced in 2 publications.
- Ludvigsen M et al. Proteomic Characterization of Colorectal Cancer Tissue from Patients Identifies Novel Putative Protein Biomarkers. Curr Issues Mol Biol 43:1043-1056 (2021). PubMed: 34563043
- Schlabritz-Loutsevitch N et al. A first case of hepatocellular carcinoma in the baboon (Papio spp.) placenta. J Med Primatol 48:68-73 (2019). PubMed: 30246873