Recombinant
RabMAb

Recombinant Anti-liver FABP antibody [EPR20464] (ab222517)

Overview

  • Product name

    Anti-liver FABP antibody [EPR20464]
    See all liver FABP primary antibodies
  • Description

    Rabbit monoclonal [EPR20464] to liver FABP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein within Human liver FABP aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P07148

  • Positive control

    • WB: Human fetal colon, HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate, human fetal liver, rat and mouse liver. IHC-P: Human liver and colon tissue. Human hepatocellular carcinoma tissue. Human colon cancer and gastric cancer tissue. Rat liver tissue. ICC/IF: HepG2 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.02% BSA, 40% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20464
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab222517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
IHC-P 1/3000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/100.

Target

  • Function

    Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport.
  • Sequence similarities

    Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • Domain

    Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • FABP 1 antibody
    • FABP1 antibody
    • FABPL antibody
    • FABPL_HUMAN antibody
    • Fatty Acid Binding Protein 1 antibody
    • Fatty acid binding protein 1 liver antibody
    • Fatty Acid Binding Protein antibody
    • Fatty acid-binding protein 1 antibody
    • Fatty acid-binding protein antibody
    • Fatty acid-binding protein liver antibody
    • L FABP antibody
    • L-FABP antibody
    • liver antibody
    • Liver-type fatty acid-binding protein antibody
    see all

Images

  • Lanes 1-4 : Anti-liver FABP antibody [EPR20464] (ab222517) at 1/1000 dilution
    Lane 5 : Anti-liver FABP antibody [EPR20464] (ab222517) at 1/2000 dilution

    Lane 1 : Human fetal colon lysate
    Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
    Lane 3 : Human fetal liver lysate
    Lane 4 : Rat liver lysate
    Lane 5 : Mouse liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 14 kDa



    Blocking buffer: 5% NFDM/TBST.

    Exposure time:

    Lanes 1-2: 1 minute

    Lane 3: 30 seconds

    Lane 4: 5 seconds

    Lane 5: 1 second

    The molecular weight observed is consistent with the literature (PMID: 21504868). 

  • Immunohistochemical analysis of paraffin-embedded human liver tissue labeling liver FABP with ab222517 at 1/3000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on human hepatocytes and sinusoids (PMID: 3123629). Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

     

  • Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling liver FABP with ab222517 at 1/3000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic and weak nuclear staining on human hepatocellular carcinoma. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

     

  • Immunohistochemical analysis of paraffin-embedded human colon tissue labeling liver FABP with ab222517 at 1/3000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic and weak nuclear staining on human colon (PMID: 15138477). Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

     

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling liver FABP with ab222517 at 1/3000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic and nuclear staining on human colon cancer (PMID: 15138477). Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

     

  • Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling liver FABP with ab222517 at 1/3000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear and cytoplasmic staining on human gastric cancer (PMID: 15051923). Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

  • Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling liver FABP with ab222517 at 1/3000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear and cytoplasmic staining on rat liver is observed. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

  • Immunofluorescent analysis of 4% paraformaldehyde fixed, 0.1% tritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling liver FABP with ab222517 at 1/100 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear and cytoplasmic staining on HepG2 cell line. Nuclear counterstain DAPI (blue). Counterstain Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (red).

    The negative control was secondary antibody only.

References

ab222517 has not yet been referenced specifically in any publications.

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