Recombinant
RabMAb

Recombinant Anti-liver FABP antibody [EPR5895(2)] (ab129203)

Overview

  • Product name

    Anti-liver FABP antibody [EPR5895(2)]
    See all liver FABP primary antibodies
  • Description

    Rabbit monoclonal [EPR5895(2)] to liver FABP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human liver FABP aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Fetal liver, Mouse liver, Human small intestine and HepG2 whole cell lysate (ab7900); Permeabilized HepG2 cells.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 4.55 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR5895(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab129203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport.
    • Sequence similarities

      Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
    • Domain

      Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • FABP 1 antibody
      • FABP1 antibody
      • FABPL antibody
      • FABPL_HUMAN antibody
      • Fatty Acid Binding Protein 1 antibody
      • Fatty acid binding protein 1 liver antibody
      • Fatty Acid Binding Protein antibody
      • Fatty acid-binding protein 1 antibody
      • Fatty acid-binding protein antibody
      • Fatty acid-binding protein liver antibody
      • L FABP antibody
      • L-FABP antibody
      • liver antibody
      • Liver-type fatty acid-binding protein antibody
      see all

    Images

    • All lanes : Anti-liver FABP antibody [EPR5895(2)] (ab129203) at 1/1000 dilution

      Lane 1 : Fetal liver lysate
      Lane 2 : Mouse liver lysate
      Lane 3 : Human small intestine lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 14 kDa

    • ab129203, at 1/10 dilution, staining FABP in permeabilized HepG2 cells by Flow cytometry (Red). Green represents rabbit IgG negative control.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    See all 3 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Pig Tissue lysate - whole (Liver)
    Gel Running Conditions
    Reduced Denaturing (13)
    Loading amount
    20 µg
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted May 19 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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