Product nameAnti-Livin antibody [51.3_2A3]
See all Livin primary antibodies
DescriptionMouse monoclonal [51.3_2A3] to Livin
Specificityab127979 specificity has been determined by protein microarray.
Tested applicationsSuitable for: WB, IP, ICC/IFmore details
Species reactivityReacts with: Human
Tissue, cells or virus corresponding to Human Livin.
- ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab127979 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 33 kDa.|
|IP||Use a concentration of 4 µg/ml. per reaction|
FunctionProtects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine. Suppression of apoptosis is mediated by activation of MAPK8/JNK1, and possibly also of MAPK9/JNK2. This activation depends on TAB1 and NR2C2/TAK1. In vitro, inhibits caspase-3 and proteolytic activation of pro-caspase-9. Isoform 1 blocks staurosporine-induced apoptosis. Isoform 2 blocks etoposide-induced apoptosis.
Tissue specificityIsoform 1 and isoform 2 are expressed at very low levels or not detectable in most adult tissues. Detected in adult heart, placenta, lung, lymph node, spleen and ovary, and in several carcinoma cell lines. Isoform 2 is detected in fetal kidney, heart and spleen, and at lower levels in adult brain, skeletal muscle and peripheral blood leukocytes.
Sequence similaritiesBelongs to the IAP family.
Contains 1 BIR repeat.
Contains 1 RING-type zinc finger.
Cellular localizationNucleus. Cytoplasm. Nuclear, and in a filamentous pattern throughout the cytoplasm.
- Information by UniProt
- Baculoviral IAP repeat containing 7 antibody
- Baculoviral IAP repeat containing protein 7 antibody
- Baculoviral IAP repeat-containing protein 7 antibody
Lanes 1-3 : Anti-Livin antibody [51.3_2A3] (ab127979) at 1/1000 dilution
Lanes 4-5 : V5 antibody control at 1/1000 dilution
Lane 6 : V5 antibody control at 1/1000 dilution
Lanes 1 & 4 : Transfected HeLa cell lysate
Lanes 2 & 5 : Non-Transfected HeLa cell lysate
Lanes 3 & 6 : SH-SY5Y cell lysate
Predicted band size: 33 kDa
ab127979 at 1/100 staining Livin in 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized HeLa cells by Immunocytochemistry.
2 µg of ab127979 was added to aliquots of cleared cell lysates from transfected HeLa cells and incubated for 2 hr at 4°C. Protein-G Dynabeads were then added, and lysates were incubated for additional 2 hr at 4°C to capture the IgG-protein complex. Anti-V5 antibody was used in parallel as a positive control. As a negative control, primary antibody was excluded.
Lane 1: Transfected HeLa Cell Lysate control
Lane 2: Transfected HeLa Cell lysate with ab127979
Lane 3: Transfected HeLa Cell lysate without ab127979
Lane 4: V5 tag control
ab127979 has not yet been referenced specifically in any publications.