Overview

  • Product name

    Anti-Livin antibody [51.3_2A3]
    See all Livin primary antibodies
  • Description

    Mouse monoclonal [51.3_2A3] to Livin
  • Host species

    Mouse
  • Specificity

    ab127979 specificity has been determined by protein microarray.
  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human Livin.

  • Positive control

    • ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab127979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 33 kDa.
IP Use a concentration of 4 µg/ml. per reaction
ICC/IF 1/100.

Target

  • Function

    Protects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine. Suppression of apoptosis is mediated by activation of MAPK8/JNK1, and possibly also of MAPK9/JNK2. This activation depends on TAB1 and NR2C2/TAK1. In vitro, inhibits caspase-3 and proteolytic activation of pro-caspase-9. Isoform 1 blocks staurosporine-induced apoptosis. Isoform 2 blocks etoposide-induced apoptosis.
  • Tissue specificity

    Isoform 1 and isoform 2 are expressed at very low levels or not detectable in most adult tissues. Detected in adult heart, placenta, lung, lymph node, spleen and ovary, and in several carcinoma cell lines. Isoform 2 is detected in fetal kidney, heart and spleen, and at lower levels in adult brain, skeletal muscle and peripheral blood leukocytes.
  • Sequence similarities

    Belongs to the IAP family.
    Contains 1 BIR repeat.
    Contains 1 RING-type zinc finger.
  • Cellular localization

    Nucleus. Cytoplasm. Nuclear, and in a filamentous pattern throughout the cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Baculoviral IAP repeat containing 7 antibody
    • Baculoviral IAP repeat containing protein 7 antibody
    • Baculoviral IAP repeat-containing protein 7 antibody
    • BIRC 7 antibody
    • birc7 antibody
    • BIRC7_HUMAN antibody
    • KIAP antibody
    • Kidney inhibitor of apoptosis protein antibody
    • Livin antibody
    • Livin inhibitor of apotosis antibody
    • Melanoma inhibitor of apoptosis protein antibody
    • ML IAP antibody
    • ML-IAP antibody
    • MLIAP antibody
    • RING finger protein 50 antibody
    • RNF 50 antibody
    • RNF50 antibody
    see all

Images

  • Lanes 1-3 : Anti-Livin antibody [51.3_2A3] (ab127979) at 1/1000 dilution
    Lanes 4-5 : V5 antibody control at 1/1000 dilution
    Lane 6 : V5 antibody control at 1/1000 dilution

    Lanes 1 & 4 : Transfected HeLa cell lysate
    Lanes 2 & 5 : Non-Transfected HeLa cell lysate
    Lanes 3 & 6 : SH-SY5Y cell lysate

    Predicted band size: 33 kDa

  • ab127979 at 1/100 staining Livin in 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized HeLa cells by Immunocytochemistry.
  • 2 µg of ab127979 was added to aliquots of cleared cell lysates from transfected HeLa cells and incubated for 2 hr at 4°C. Protein-G Dynabeads were then added, and lysates were incubated for additional 2 hr at 4°C to capture the IgG-protein complex. Anti-V5 antibody was used in parallel as a positive control. As a negative control, primary antibody was excluded.
    Lane 1: Transfected HeLa Cell Lysate control
    Lane 2: Transfected HeLa Cell lysate with ab127979
    Lane 3: Transfected HeLa Cell lysate without ab127979
    Lane 4: V5 tag control

References

ab127979 has not yet been referenced specifically in any publications.

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