Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-LKB1 antibody [EPR19379] (ab199970)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19379] to LKB1
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-LKB1 antibody [EPR19379]
    See all LKB1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19379] to LKB1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human LKB1 aa 1-350. The exact sequence is proprietary.
    Database link: Q15831

  • Positive control

    • WB: Jurkat and K562 whole cell lysates; Human brain, fetal heart, fetal kidney and fetal testis lysates. IP: K562 and human testis lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab199970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 48, 50 kDa (predicted molecular weight: 45, 49 kDa).
IP 1/100.

Target

  • Function

    Essential role in G1 cell cycle arrest. Phosphorylates and activates members of the AMPK-related subfamily of protein kinases. Tumor suppressor.

  • Tissue specificity

    Ubiquitously expressed. Strongest expression in testis and fetal liver.

  • Involvement in disease

    Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) [MIM:175200]. PJS is a rare hereditary disease in which there is predisposition to benign and malignant tumors of many organ systems. PJS is an autosomal dominant disorder characterized by melanocytic macules of the lips, multiple gastrointestinal hamartomatous polyps and an increased risk for various neoplasms, including gastrointestinal cancer.
    Defects in STK11 have been associated with testicular tumors (TEST) [MIM:273300]. A common solid malignancy in males. Germ cell tumors of the testis constitute 95% of all testicular neoplasms.

  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. LKB1 subfamily.
    Contains 1 protein kinase domain.

  • Post-translational
    modifications

    Phosphorylated by a cAMP-dependent protein kinase.

  • Cellular localization

    Nucleus. Cytoplasm. Relocates to the cytoplasm when bound to CAB39 and STRAD or CAB39 and ALS2CR2.
  • Information by UniProt

  • Database links

    • Alternative names

      • hLKB1 antibody
      • Liver kinase B1 antibody
      • LKB1 antibody
      • PJS antibody
      • Polarization related protein LKB1 antibody
      • Renal carcinoma antigen NY-REN-19 antibody
      • Serine/Threonine Kinase 11 antibody
      • Serine/threonine protein kinase 11 antibody
      • Serine/threonine protein kinase LKB1 antibody
      • Serine/threonine protein kinase STK11 antibody
      • Serine/threonine-protein kinase 11 antibody
      • Serine/threonine-protein kinase LKB1 antibody
      • Serine/threonine-protein kinase XEEK1 antibody
      • Stk11 antibody
      • STK11_HUMAN antibody
      see all

    Images

    • Western blot - Anti-LKB1 antibody [EPR19379] (ab199970)
      Western blot - Anti-LKB1 antibody [EPR19379] (ab199970)

      Lane 1: Wild-type HAP1 whole cell lysate (20 µg) 
      Lane 2: STK11 knockout HAP1 whole cell lysate (20 µg) 
      Lane 3: Hu Testis whole cell lysate (20 µg) 

      Lanes 1 - 3: Merged signal (red and green). Green - ab199970 observed at 50 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab199970 was shown to specifically react with STK11 in wild-type HAP1 cells. No band was observed when STK11 knockout samples were examined. Wild-type and STK11 knockout samples were subjected to SDS-PAGE.  Ab199970 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-LKB1 antibody [EPR19379] (ab199970)
      Western blot - Anti-LKB1 antibody [EPR19379] (ab199970)
      All lanes : Anti-LKB1 antibody [EPR19379] (ab199970) at 1/1000 dilution

      Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
      Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
      Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 45, 49 kDa
      Observed band size: 50 kDa
      why is the actual band size different from the predicted?


      Exposure time: 15 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

      Negative control: HeLa (PMID 11297520; PMID24086281; PMID 19340305).

    • Western blot - Anti-LKB1 antibody [EPR19379] (ab199970)
      Western blot - Anti-LKB1 antibody [EPR19379] (ab199970)
      All lanes : Anti-LKB1 antibody [EPR19379] (ab199970) at 1/1000 dilution

      Lane 1 : Human brain lysate
      Lane 2 : Human fetal heart lysate
      Lane 3 : Human fetal kidney lysate
      Lane 4 : Human fetal testis lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

      Predicted band size: 45, 49 kDa
      Observed band size: 50 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

       

    • Immunoprecipitation - Anti-LKB1 antibody [EPR19379] (ab199970)
      Immunoprecipitation - Anti-LKB1 antibody [EPR19379] (ab199970)

      LKB1 was immunoprecipitated from 1 mg of K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with ab199970 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab199970 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

      Lane 1: K562  whole cell lysate, 10 μg (Input).

      Lane 2: ab199970 IP in K562  whole cell lysate.

      Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab199970 in K562 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 minutes.

      LKB1 isoforms: PMID 18854309.

    • Immunoprecipitation - Anti-LKB1 antibody [EPR19379] (ab199970)
      Immunoprecipitation - Anti-LKB1 antibody [EPR19379] (ab199970)

      LKB1 was immunoprecipitated from 1 mg of Human testis lysate with ab199970 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab199970 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Lane 1: Human testis lysate, 10 μg (Input).

      Lane 2: ab199970 IP in Human testis lysate .

      Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab199970 in Human testis lysate .

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 minutes.

      LKB1 isoforms: PMID 18854309.

    References (3)

    Publishing research using ab199970? Please let us know so that we can cite the reference in this datasheet.

    ab199970 has been referenced in 3 publications.

    • Li Y  et al. CD36 plays a negative role in the regulation of lipophagy in hepatocytes through an AMPK-dependent pathway. J Lipid Res N/A:N/A (2019). PubMed: 30662007
    • Zhang H  et al. Genistein protects against ox-LDL-induced senescence through enhancing SIRT1/LKB1/AMPK-mediated autophagy flux in HUVECs. Mol Cell Biochem N/A:N/A (2018). PubMed: 30443855
    • Qiu B  et al. EMT induced by loss of LKB1 promotes migration and invasion of liver cancer cells through ZEB1-induced YAP signaling. Oncol Lett 16:6465-6471 (2018). PubMed: 30405784

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