Anti-LKB1 antibody [Ley 37D/G6] (ab15095)
![Western blot - Anti-LKB1 antibody [Ley 37D/G6] (ab15095)](https://www.abcam.com/ps/products/15/ab15095/Images/ab15095-254244-anti-lkb1-antibody-ley-37d-g6-western-blot.jpg "Western blot - Anti-LKB1 antibody [Ley 37D/G6] (ab15095)")
Key features and details
- Mouse monoclonal [Ley 37D/G6] to LKB1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-LKB1 antibody [Ley 37D/G6]
See all LKB1 primary antibodies -
Description
Mouse monoclonal [Ley 37D/G6] to LKB1 -
Host species
Mouse -
Specificity
This antibody detects a single clean band representing LKB1/STK11 in Western blots on cells expressing LKB1. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to LKB1.
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Positive control
- HAP1 and MEF cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
Ley 37D/G6 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab15095 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (6) |
1/100. Detects a band of approximately 50 kDa (predicted molecular weight: 48.6 kDa).
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| Notes |
|---|
|
WB
1/100. Detects a band of approximately 50 kDa (predicted molecular weight: 48.6 kDa). |
Target
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Function
Essential role in G1 cell cycle arrest. Phosphorylates and activates members of the AMPK-related subfamily of protein kinases. Tumor suppressor. -
Tissue specificity
Ubiquitously expressed. Strongest expression in testis and fetal liver. -
Involvement in disease
Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) [MIM:175200]. PJS is a rare hereditary disease in which there is predisposition to benign and malignant tumors of many organ systems. PJS is an autosomal dominant disorder characterized by melanocytic macules of the lips, multiple gastrointestinal hamartomatous polyps and an increased risk for various neoplasms, including gastrointestinal cancer.
Defects in STK11 have been associated with testicular tumors (TEST) [MIM:273300]. A common solid malignancy in males. Germ cell tumors of the testis constitute 95% of all testicular neoplasms. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. LKB1 subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated by a cAMP-dependent protein kinase. -
Cellular localization
Nucleus. Cytoplasm. Relocates to the cytoplasm when bound to CAB39 and STRAD or CAB39 and ALS2CR2. - Information by UniProt
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Database links
- Entrez Gene: 6794 Human
- Omim: 602216 Human
- SwissProt: Q15831 Human
- Unigene: 515005 Human
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Alternative names
- hLKB1 antibody
- Liver kinase B1 antibody
- LKB1 antibody
see all
Images
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Western blot - Anti-LKB1 antibody [Ley 37D/G6] (ab15095)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: LKB1 knockout HAP1 cell lysate (20 µg)Lanes 1 and 2: Merged signal (red and green). Green - ab15095 observed at 50 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab15095 was shown to recognize LKB1 when LKB1 knockout samples were used, along with additional cross-reactive bands. Wild-type and LKB1 knockout samples were subjected to SDS-PAGE. ab15095 and ab181602 (loading control to LKB1) were diluted 1/100 and 1/10 000 and incubated overnight at 4°C. Blots were developed withGoat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging. -
![Western blot - Anti-LKB1 antibody [Ley 37D/G6] (ab15095)](https://www.abcam.com/ps/products/15/ab15095/Images/ab15095-264645-anti-lkb1-antibody-ley-37d-g6-western-blot.png)
Western blot - Anti-LKB1 antibody [Ley 37D/G6] (ab15095)Image courtesy of anonymous abreviewerAll lanes : Anti-LKB1 antibody [Ley 37D/G6] (ab15095) at 1/1000 dilution
All lanes :
Secondary
All lanes : Polyclonal goat anti-mouse conjugated with horse radish peroxidase.
Predicted band size: 48.6 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?Negative control (Lane 1): LKB1 knockout MEF cells.
Positive control (Lane 2): wildtype MEF cells.
Blocking step: 5% milk as blocking agent for 1 hour at 25 °C.
Incubation: 16 hours at 4 °C, using 5% milk in TBS/0.1 Tween as diluent.
References (34)
ab15095 has been referenced in 34 publications.
- Kitajima S et al. Suppression of STING Associated with LKB1 Loss in KRAS-Driven Lung Cancer. Cancer Discov 9:34-45 (2019). PubMed: 30297358
- Ollila S et al. Stromal Lkb1 deficiency leads to gastrointestinal tumorigenesis involving the IL-11-JAK/STAT3 pathway. J Clin Invest 128:402-414 (2018). PubMed: 29202476
- Hong B et al. Activation of the LKB1-SIK1 signaling pathway inhibits the TGF-ß-mediated epithelial-mesenchymal transition and apoptosis resistance of ovarian carcinoma cells. Mol Med Rep 17:2837-2844 (2018). PubMed: 29257268
- Ding Z et al. PCSK9 expression in the ischaemic heart and its relationship to infarct size, cardiac function, and development of autophagy. Cardiovasc Res 114:1738-1751 (2018). PubMed: 29800228
- Mo J et al. Revealing the immune perturbation of black phosphorus nanomaterials to macrophages by understanding the protein corona. Nat Commun 9:2480 (2018). PubMed: 29946125
