Validated using a knockout cell line

Anti-LKB1 antibody [Ley 37D/G6] (ab15095)

Reviews (12)Q&A (9)References (32)

Overview

  • Product name
    Anti-LKB1 antibody [Ley 37D/G6]
    See all LKB1 primary antibodies
  • Description
    Mouse monoclonal [Ley 37D/G6] to LKB1
  • Host species
    Mouse
  • Specificity
    This antibody detects a single clean band representing LKB1/STK11 in Western blots on cells expressing LKB1.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein corresponding to LKB1.

  • Positive control
    • Adenocarcinoma H441 cell line, Lung carcinoma cell line H1299

Properties

Applications

Our Abpromise guarantee covers the use of ab15095 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100. Detects a band of approximately 50 kDa (predicted molecular weight: 48.6 kDa).

Target

  • Function
    Essential role in G1 cell cycle arrest. Phosphorylates and activates members of the AMPK-related subfamily of protein kinases. Tumor suppressor.
  • Tissue specificity
    Ubiquitously expressed. Strongest expression in testis and fetal liver.
  • Involvement in disease
    Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) [MIM:175200]. PJS is a rare hereditary disease in which there is predisposition to benign and malignant tumors of many organ systems. PJS is an autosomal dominant disorder characterized by melanocytic macules of the lips, multiple gastrointestinal hamartomatous polyps and an increased risk for various neoplasms, including gastrointestinal cancer.
    Defects in STK11 have been associated with testicular tumors (TEST) [MIM:273300]. A common solid malignancy in males. Germ cell tumors of the testis constitute 95% of all testicular neoplasms.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. LKB1 subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated by a cAMP-dependent protein kinase.
  • Cellular localization
    Nucleus. Cytoplasm. Relocates to the cytoplasm when bound to CAB39 and STRAD or CAB39 and ALS2CR2.
  • Information by UniProt
  • Database links
    • Alternative names
      • hLKB1 antibody
      • Liver kinase B1 antibody
      • LKB1 antibody
      • PJS antibody
      • Polarization related protein LKB1 antibody
      • Renal carcinoma antigen NY-REN-19 antibody
      • Serine/Threonine Kinase 11 antibody
      • Serine/threonine protein kinase 11 antibody
      • Serine/threonine protein kinase LKB1 antibody
      • Serine/threonine protein kinase STK11 antibody
      • Serine/threonine-protein kinase 11 antibody
      • Serine/threonine-protein kinase LKB1 antibody
      • Serine/threonine-protein kinase XEEK1 antibody
      • Stk11 antibody
      • STK11_HUMAN antibody
      see all

    Images

    • Western blot - Anti-LKB1 antibody [Ley 37D/G6] (ab15095)
      Western blot - Anti-LKB1 antibody [Ley 37D/G6] (ab15095)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: LKB1 knockout HAP1 cell lysate (20 µg)

      Lanes 1 and 2: Merged signal (red and green). Green - ab15095 observed at 50 kDa. Red - loading control, ab181602, observed at 37 kDa.
      ab15095 was shown to recognize LKB1 when LKB1 knockout samples were used, along with additional cross-reactive bands. Wild-type and LKB1 knockout samples were subjected to SDS-PAGE. ab15095 and ab181602 (loading control to LKB1) were diluted 1/100 and 1/10 000 and incubated overnight at 4°C. Blots were developed withGoat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Western blot - Anti-LKB1 antibody [Ley 37D/G6] (ab15095)
      Western blot - Anti-LKB1 antibody [Ley 37D/G6] (ab15095)Image courtesy of anonymous abreviewer
      All lanes : Anti-LKB1 antibody [Ley 37D/G6] (ab15095) at 1/1000 dilution

      All lanes :

      Secondary
      All lanes : Polyclonal goat anti-mouse conjugated with horse radish peroxidase.

      Predicted band size: 48.6 kDa
      Observed band size: 55 kDa
      why is the actual band size different from the predicted?



      Negative control (Lane 1): LKB1 knockout MEF cells.

      Positive control (Lane 2): wildtype MEF cells.

      Blocking step: 5% milk as blocking agent for 1 hour at 25 °C.

      Incubation: 16 hours at 4 °C, using 5% milk in TBS/0.1 Tween as diluent.

       

      See Abreview

    References

    This product has been referenced in:
    • Mo J  et al. Revealing the immune perturbation of black phosphorus nanomaterials to macrophages by understanding the protein corona. Nat Commun 9:2480 (2018). Read more (PubMed: 29946125) »
    • Ollila S  et al. Stromal Lkb1 deficiency leads to gastrointestinal tumorigenesis involving the IL-11-JAK/STAT3 pathway. J Clin Invest 128:402-414 (2018). Read more (PubMed: 29202476) »
    See all 32 Publications for this product

    Publishing research using ab15095? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-10 of 21 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-LKB1 antibody [Ley 37D/G6]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Lung adenocarcinoma)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 8.5 buffer
    Permeabilization
    No
    Specification
    Lung adenocarcinoma
    Fixative
    Formaldehyde
    Read More

    Mr. Allan Wang

    Verified customer

    Submitted Mar 20 2018

    Western blot abreview for Anti-LKB1 antibody [Ley 37D/G6]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (A549)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris nupage gel)
    Loading amount
    10 µg
    Specification
    A549
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 12 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-LKB1 antibody [Ley 37D/G6]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Hepatocarcinoma (HCC))
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer ph 6, 20 min at 97ºC IN A PT LINK
    Permeabilization
    No
    Specification
    Hepatocarcinoma (HCC)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Miss. Virginia Gutiérrez

    Verified customer

    Submitted Jan 07 2016

    Western blot abreview for Anti-LKB1 antibody [Ley 37D/G6]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Mouse Cell lysate - whole cell (MEF)
    Specification
    MEF
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 10 2013

    Question

    I have a customer enquiry about antibodies to CAMKK and LKB1, application = WB / IF, species = dictyostelium

    I know that you do not have antibodies to these targets tested in Dictyostelium, but I had trouble finding out about identity of the proteins between the target species and Dicty. So I am hoping you can get some information in a more expedient fashion that I can pass onto the customer.

    I guess it might be possible that at Trial (testing) discount might be applicable?

    Read More
    Answer

    Thank you for contacting us.

    I have performed a sequence alignment for LKB1 for Dictyostelium and for human (which is used to raise most of our antibodies.) Unfortunately there is only a total of 33% homology between the two proteins. I have contacted the lab and indeed all the immunogens of the anti-LKB1antibodies guaranteed to work in WB/IF in our catalogue have a 28-50% sequence homology with the Dictyostelium protein and therefore are not likely to work.

    The amino acid sequence for LKB1 for Dictyostelium that I used can be found at http://www.uniprot.org/uniprot/Q15831.fasta. Unfortunately, this is the predicted amino acid sequence for the species and has not been confirmed therefore, if your customer has the verified sequence of this protein, then I will be happy to repeat these alignments to verify the homology.

    Regarding an anti-CAMKK antibody, I am not able to recommend a suitable one unless I have the Dictyostelium amino acid sequence for this protein. If your customer could send it, then again I will be happy to perform the sequence alignments.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    I have a customer enquiry about antibodies to CAMKK and LKB1, application = WB / IF, species = dictyostelium

    I know that you do not have antibodies to these targets tested in Dictyostelium, but I had trouble finding out about identity of the proteins between the target species and Dicty. So I am hoping you can get some information in a more expedient fashion that I can pass onto the customer.

    I guess it might be possible that at Trial (testing) discount might be applicable?

    I look forward to your feedback which I can forward to the customer.

    Read More
    Answer

    Thank you for contacting us.

    I have found the amino acid sequence for LKB1 for Dictyostelium which can be found at http://www.uniprot.org/uniprot/Q15831.fasta

    I have contacted the lab to see if any of our antibodies would be compatible for use in Dictyostelium.

    I have not been able to find the sequence for CAMKK for Dictyostelium. Could you ask your customer to send it if they have it so that I may perform the sequence alignments?

    I look forward to your reply.

    Read More

    Question

    What is the epitope for antibody ab15095?

    Read More
    Answer

    Thank you for contacting us.

    I had a quick chat with my colleague in lab. They have confirmed that the antibody has been raised against the full length protein so they do not have determined the epitope this antibody is recognizing

    I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    I wonder if you have the immunizing peptide for ab15095 (anti-Lkb1) for sale. I am planning to use the antibody for IHC-P and would like to include a negative control with a neutralized antibody to exclude false positive staining/non-specific binding. Thanks in advance for your help.

    Read More
    Answer

    Thank you for contacting us.

    I have checked with supplying the lab, but unfortunately, the immunogen is not available for sale.

    Alternatively, I could recommend an isotype control (mouse IgG2b) to be used as negative control, for instance:

    ab91366 (Mouse IgG2b [PLPV219] - Isotype Control)
    https://www.abcam.com/Mouse-IgG2b-PLPV219-Isotype-Control-ab91366.html

    ab18421 (Mouse IgG2b, kappa monoclonal [MG2b-57] - Isotype control)
    https://www.abcam.com/Mouse-IgG2b-kappa-monoclonal-MG2b-57-Isotype-control-ab18421.html

    ab18469 (Mouse IgG2b, kappa monoclonal [MPC-11] - Isotype control)
    https://www.abcam.com/Mouse-IgG2b-kappa-monoclonal-MPC-11-Isotype-control-ab18469.html

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    I wonder if you have the immunizing peptide for ab15095 (anti-Lkb1) for sale. I am planning to use the antibody for IHC-P and would like to include a negative control with a neutralized antibody to exclude false positive staining/non-specific binding. Thanks in advance for your help.

    Read More
    Answer

    Thank you for contacting us.

    The immunogen was ahighly purified recombinant full length protein made in E. coli, but is not a peptide. I will check with the lab if the immunogen might be commercially available and let you know what I find out.

    Thank you for your patience and understanding.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Western blot abreview for Anti-LKB1 antibody [Ley 37D/G6]

     Good
    Abreviews
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (neurons)
    Loading amount
    50 µg
    Specification
    neurons
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 31 2011

    1-10 of 21 Abreviews or Q&A

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