Product nameAnti-LKB1 antibody [Ley 37D/G6]
See all LKB1 primary antibodies
DescriptionMouse monoclonal [Ley 37D/G6] to LKB1
SpecificityThis antibody detects a single clean band representing LKB1/STK11 in Western blots on cells expressing LKB1.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Recombinant full length protein corresponding to LKB1.
- Adenocarcinoma H441 cell line, Lung carcinoma cell line H1299
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
Clone numberLey 37D/G6
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab15095 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100. Detects a band of approximately 50 kDa (predicted molecular weight: 48.6 kDa).|
FunctionEssential role in G1 cell cycle arrest. Phosphorylates and activates members of the AMPK-related subfamily of protein kinases. Tumor suppressor.
Tissue specificityUbiquitously expressed. Strongest expression in testis and fetal liver.
Involvement in diseaseDefects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) [MIM:175200]. PJS is a rare hereditary disease in which there is predisposition to benign and malignant tumors of many organ systems. PJS is an autosomal dominant disorder characterized by melanocytic macules of the lips, multiple gastrointestinal hamartomatous polyps and an increased risk for various neoplasms, including gastrointestinal cancer.
Defects in STK11 have been associated with testicular tumors (TEST) [MIM:273300]. A common solid malignancy in males. Germ cell tumors of the testis constitute 95% of all testicular neoplasms.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. LKB1 subfamily.
Contains 1 protein kinase domain.
modificationsPhosphorylated by a cAMP-dependent protein kinase.
Cellular localizationNucleus. Cytoplasm. Relocates to the cytoplasm when bound to CAB39 and STRAD or CAB39 and ALS2CR2.
- Information by UniProt
- hLKB1 antibody
- Liver kinase B1 antibody
- LKB1 antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: LKB1 knockout HAP1 cell lysate (20 µg)
Lanes 1 and 2: Merged signal (red and green). Green - ab15095 observed at 50 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab15095 was shown to recognize LKB1 when LKB1 knockout samples were used, along with additional cross-reactive bands. Wild-type and LKB1 knockout samples were subjected to SDS-PAGE. ab15095 and ab181602 (loading control to LKB1) were diluted 1/100 and 1/10 000 and incubated overnight at 4°C. Blots were developed withGoat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
All lanes : Anti-LKB1 antibody [Ley 37D/G6] (ab15095) at 1/1000 dilution
All lanes :
All lanes : Polyclonal goat anti-mouse conjugated with horse radish peroxidase.
Predicted band size: 48.6 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Negative control (Lane 1): LKB1 knockout MEF cells.
Positive control (Lane 2): wildtype MEF cells.
Blocking step: 5% milk as blocking agent for 1 hour at 25 °C.
Incubation: 16 hours at 4 °C, using 5% milk in TBS/0.1 Tween as diluent.
This product has been referenced in:
- Mo J et al. Revealing the immune perturbation of black phosphorus nanomaterials to macrophages by understanding the protein corona. Nat Commun 9:2480 (2018). Read more (PubMed: 29946125) »
- Ollila S et al. Stromal Lkb1 deficiency leads to gastrointestinal tumorigenesis involving the IL-11-JAK/STAT3 pathway. J Clin Invest 128:402-414 (2018). Read more (PubMed: 29202476) »