Anti-LKB1 (phospho S428) antibody (ab63473)
Key features and details
- Rabbit polyclonal to LKB1 (phospho S428)
- Suitable for: ICC/IF, WB
- Reacts with: Mouse
- Isotype: IgG
Overview
-
Product name
Anti-LKB1 (phospho S428) antibody
See all LKB1 primary antibodies -
Description
Rabbit polyclonal to LKB1 (phospho S428) -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide corresponding to Human LKB1 (phospho S428).
Database link: Q15831 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab63473 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab63473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/500 - 1/1000.
|
|
WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 48 kDa.
|
Notes |
---|
ICC/IF
1/500 - 1/1000. |
WB
1/500 - 1/1000. Predicted molecular weight: 48 kDa. |
Target
-
Function
Essential role in G1 cell cycle arrest. Phosphorylates and activates members of the AMPK-related subfamily of protein kinases. Tumor suppressor. -
Tissue specificity
Ubiquitously expressed. Strongest expression in testis and fetal liver. -
Involvement in disease
Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) [MIM:175200]. PJS is a rare hereditary disease in which there is predisposition to benign and malignant tumors of many organ systems. PJS is an autosomal dominant disorder characterized by melanocytic macules of the lips, multiple gastrointestinal hamartomatous polyps and an increased risk for various neoplasms, including gastrointestinal cancer.
Defects in STK11 have been associated with testicular tumors (TEST) [MIM:273300]. A common solid malignancy in males. Germ cell tumors of the testis constitute 95% of all testicular neoplasms. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. LKB1 subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated by a cAMP-dependent protein kinase. -
Cellular localization
Nucleus. Cytoplasm. Relocates to the cytoplasm when bound to CAB39 and STRAD or CAB39 and ALS2CR2. - Information by UniProt
-
Database links
- Entrez Gene: 20869 Mouse
- SwissProt: Q9WTK7 Mouse
-
Alternative names
- hLKB1 antibody
- Liver kinase B1 antibody
- LKB1 antibody
see all
Images
-
All lanes : Anti-LKB1 (phospho S428) antibody (ab63473) at 1/500 dilution
Lane 1 : mouse brain extracts
Lane 2 : mouse brain extracts with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands. -
ab63473 at 1/500 dilution staining LKB1 in NIH3T3 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (8)
ab63473 has been referenced in 8 publications.
- Zhang J et al. PSPH induces cell autophagy and promotes cell proliferation and invasion in the hepatocellular carcinoma cell line Huh7 via the AMPK/mTOR/ULK1 signaling pathway. Cell Biol Int 45:305-319 (2021). PubMed: 33079432
- Wu J et al. Ferulic Acid Ameliorates Hepatic Inflammation and Fibrotic Liver Injury by Inhibiting PTP1B Activity and Subsequent Promoting AMPK Phosphorylation. Front Pharmacol 12:754976 (2021). PubMed: 34566665
- Kinsey CG et al. Protective autophagy elicited by RAF?MEK?ERK inhibition suggests a treatment strategy for RAS-driven cancers. Nat Med 25:620-627 (2019). PubMed: 30833748
- Hong B et al. Activation of the LKB1-SIK1 signaling pathway inhibits the TGF-ß-mediated epithelial-mesenchymal transition and apoptosis resistance of ovarian carcinoma cells. Mol Med Rep 17:2837-2844 (2018). PubMed: 29257268
- Ding Z et al. PCSK9 expression in the ischaemic heart and its relationship to infarct size, cardiac function, and development of autophagy. Cardiovasc Res 114:1738-1751 (2018). PubMed: 29800228
- Sueda T et al. BRAF V600E inhibition stimulates AMP-activated protein kinase-mediated autophagy in colorectal cancer cells. Sci Rep 6:18949 (2016). PubMed: 26750638
- Yang SH et al. Lovastatin overcomes gefitinib resistance through TNF-a signaling in human cholangiocarcinomas with different LKB1 statuses in vitro and in vivo. Oncotarget 6:23857-73 (2015). WB, IHC . PubMed: 26160843
- Konno H et al. Cyclic Dinucleotides Trigger ULK1 (ATG1) Phosphorylation of STING to Prevent Sustained Innate Immune Signaling. Cell 155:688-98 (2013). PubMed: 24119841