• Product name

    Anti-LKB1 (phospho S428) antibody
    See all LKB1 primary antibodies
  • Description

    Rabbit polyclonal to LKB1 (phospho S428)
  • Host species

  • Specificity

    ab63473 detects endogenous levels of LKB1 only when phosphorylated at serine 428.
  • Tested applications

    Suitable for: ELISA, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic phosphopeptide derived from human LKB1 around the phosphorylation site of serine 428 (R-L-SP-A-C).

  • Positive control

    • WB: Mouse brain extracts. ICC/IF: NIH3T3 cells.



Our Abpromise guarantee covers the use of ab63473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/40000.
ICC/IF 1/500 - 1/1000.
WB 1/500 - 1/1000. Predicted molecular weight: 48 kDa.


  • Function

    Essential role in G1 cell cycle arrest. Phosphorylates and activates members of the AMPK-related subfamily of protein kinases. Tumor suppressor.
  • Tissue specificity

    Ubiquitously expressed. Strongest expression in testis and fetal liver.
  • Involvement in disease

    Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) [MIM:175200]. PJS is a rare hereditary disease in which there is predisposition to benign and malignant tumors of many organ systems. PJS is an autosomal dominant disorder characterized by melanocytic macules of the lips, multiple gastrointestinal hamartomatous polyps and an increased risk for various neoplasms, including gastrointestinal cancer.
    Defects in STK11 have been associated with testicular tumors (TEST) [MIM:273300]. A common solid malignancy in males. Germ cell tumors of the testis constitute 95% of all testicular neoplasms.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. LKB1 subfamily.
    Contains 1 protein kinase domain.
  • Post-translational

    Phosphorylated by a cAMP-dependent protein kinase.
  • Cellular localization

    Nucleus. Cytoplasm. Relocates to the cytoplasm when bound to CAB39 and STRAD or CAB39 and ALS2CR2.
  • Information by UniProt
  • Database links

  • Alternative names

    • hLKB1 antibody
    • Liver kinase B1 antibody
    • LKB1 antibody
    • PJS antibody
    • Polarization related protein LKB1 antibody
    • Renal carcinoma antigen NY-REN-19 antibody
    • Serine/Threonine Kinase 11 antibody
    • Serine/threonine protein kinase 11 antibody
    • Serine/threonine protein kinase LKB1 antibody
    • Serine/threonine protein kinase STK11 antibody
    • Serine/threonine-protein kinase 11 antibody
    • Serine/threonine-protein kinase LKB1 antibody
    • Serine/threonine-protein kinase XEEK1 antibody
    • Stk11 antibody
    • STK11_HUMAN antibody
    see all


  • All lanes : Anti-LKB1 (phospho S428) antibody (ab63473) at 1/500 dilution

    Lane 1 : mouse brain extracts
    Lane 2 : mouse brain extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 48 kDa
    Observed band size: 48 kDa
    Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.

  • ab63473 at 1/500 dilution staining LKB1 in NIH3T3 cells by Immunofluorescence, in the absence or presence of the immunising peptide.


This product has been referenced in:

  • Hong B  et al. Activation of the LKB1-SIK1 signaling pathway inhibits the TGF-ß-mediated epithelial-mesenchymal transition and apoptosis resistance of ovarian carcinoma cells. Mol Med Rep 17:2837-2844 (2018). Read more (PubMed: 29257268) »
  • Sueda T  et al. BRAF V600E inhibition stimulates AMP-activated protein kinase-mediated autophagy in colorectal cancer cells. Sci Rep 6:18949 (2016). Read more (PubMed: 26750638) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A


Thank you for contacting us.

The link to the database entry for the human LKB1 protein is:


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!

Read More
Western blot
Mouse Tissue lysate - whole (Mouse brain)
Loading amount
50 µg
Mouse brain
Gel Running Conditions
Reduced Denaturing (10-20% tris-tricine)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Miss. Anna Lauer

Verified customer

Submitted May 12 2011

For licensing inquiries, please contact partnerships@abcam.com

Sign up