Anti-LKB1 (phospho S428) antibody - C-terminal (ab138386)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-LKB1 (phospho S428) antibody - C-terminal
See all LKB1 primary antibodies -
Description
Rabbit polyclonal to LKB1 (phospho S428) - C-terminal -
Host species
Rabbit -
Specificity
ab138386 detects endogenous levels of LKB1 only when phosphorylated at Ser428. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide corresponding to residues around phospho-Ser428 from the C-terminal region of Human LKB1.
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Positive control
- CV-1 cell lysate - forskolin treated; Human breast carcinoma tissue
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49.1% PBS, 50% Glycerol, 0.88% Sodium chloride
Note: PBS without Mg2+ or Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab138386 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab138386 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 48 kDa. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Essential role in G1 cell cycle arrest. Phosphorylates and activates members of the AMPK-related subfamily of protein kinases. Tumor suppressor. -
Tissue specificity
Ubiquitously expressed. Strongest expression in testis and fetal liver. -
Involvement in disease
Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) [MIM:175200]. PJS is a rare hereditary disease in which there is predisposition to benign and malignant tumors of many organ systems. PJS is an autosomal dominant disorder characterized by melanocytic macules of the lips, multiple gastrointestinal hamartomatous polyps and an increased risk for various neoplasms, including gastrointestinal cancer.
Defects in STK11 have been associated with testicular tumors (TEST) [MIM:273300]. A common solid malignancy in males. Germ cell tumors of the testis constitute 95% of all testicular neoplasms. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. LKB1 subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated by a cAMP-dependent protein kinase. -
Cellular localization
Nucleus. Cytoplasm. Relocates to the cytoplasm when bound to CAB39 and STRAD or CAB39 and ALS2CR2. - Information by UniProt
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Database links
- Entrez Gene: 6794 Human
- Entrez Gene: 20869 Mouse
- Omim: 602216 Human
- SwissProt: Q15831 Human
- SwissProt: Q9WTK7 Mouse
- Unigene: 515005 Human
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Alternative names
- hLKB1 antibody
- Liver kinase B1 antibody
- LKB1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LKB1 (phospho S428) antibody - C-terminal (ab138386)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling LKB1 (phospho S428) with ab138386 at 1/50. -
Western blot - Anti-LKB1 (phospho S428) antibody - C-terminal (ab138386)Anti-LKB1 (phospho S428) antibody - C-terminal (ab138386) at 1/500 dilution + CV-1 cell lysate - forskolin treated at 30 µg
Predicted band size: 48 kDa
Datasheets and documents
References
This product has been referenced in:
- Li F et al. BET inhibitor JQ1 suppresses cell proliferation via inducing autophagy and activating LKB1/AMPK in bladder cancer cells. Cancer Med 8:4792-4805 (2019). Read more (PubMed: 31250978) »
