Key features and details
- Rabbit polyclonal to LKB1 (phospho S428) - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LKB1 (phospho S428) antibody - C-terminal
See all LKB1 primary antibodies
DescriptionRabbit polyclonal to LKB1 (phospho S428) - C-terminal
Specificityab138386 detects endogenous levels of LKB1 only when phosphorylated at Ser428.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide corresponding to residues around phospho-Ser428 from the C-terminal region of Human LKB1.
- CV-1 cell lysate - forskolin treated; Human breast carcinoma tissue
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49.1% PBS, 50% Glycerol, 0.88% Sodium chloride
PBS without Mg2+ or Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab138386 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Our Abpromise guarantee covers the use of ab138386 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 48 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionEssential role in G1 cell cycle arrest. Phosphorylates and activates members of the AMPK-related subfamily of protein kinases. Tumor suppressor.
Tissue specificityUbiquitously expressed. Strongest expression in testis and fetal liver.
Involvement in diseaseDefects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) [MIM:175200]. PJS is a rare hereditary disease in which there is predisposition to benign and malignant tumors of many organ systems. PJS is an autosomal dominant disorder characterized by melanocytic macules of the lips, multiple gastrointestinal hamartomatous polyps and an increased risk for various neoplasms, including gastrointestinal cancer.
Defects in STK11 have been associated with testicular tumors (TEST) [MIM:273300]. A common solid malignancy in males. Germ cell tumors of the testis constitute 95% of all testicular neoplasms.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. LKB1 subfamily.
Contains 1 protein kinase domain.
modificationsPhosphorylated by a cAMP-dependent protein kinase.
Cellular localizationNucleus. Cytoplasm. Relocates to the cytoplasm when bound to CAB39 and STRAD or CAB39 and ALS2CR2.
- Information by UniProt
- hLKB1 antibody
- Liver kinase B1 antibody
- LKB1 antibody
ab138386 has been referenced in 1 publication.
- Li F et al. BET inhibitor JQ1 suppresses cell proliferation via inducing autophagy and activating LKB1/AMPK in bladder cancer cells. Cancer Med 8:4792-4805 (2019). PubMed: 31250978