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Cell Biology Cell Cycle Cell Cycle Inhibitors p53
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Anti-LKB1 (phospho S428) antibody - C-terminal (ab138386)

  • Datasheet
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LKB1 (phospho S428) antibody - C-terminal (ab138386)
  • Western blot - Anti-LKB1 (phospho S428) antibody - C-terminal (ab138386)

Key features and details

  • Rabbit polyclonal to LKB1 (phospho S428) - C-terminal
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-LKB1 (phospho S428) antibody - C-terminal
    See all LKB1 primary antibodies
  • Description

    Rabbit polyclonal to LKB1 (phospho S428) - C-terminal
  • Host species

    Rabbit
  • Specificity

    ab138386 detects endogenous levels of LKB1 only when phosphorylated at Ser428.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to residues around phospho-Ser428 from the C-terminal region of Human LKB1.

  • Positive control

    • CV-1 cell lysate - forskolin treated; Human breast carcinoma tissue
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49.1% PBS, 50% Glycerol, 0.88% Sodium chloride

    PBS without Mg2+ or Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab138386 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • p53
    • Cancer
    • Cell cycle
    • Cell cycle inhibitors
    • p53 pathway
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • p53 pathway
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human LKB1 protein (ab119736)

Applications

Our Abpromise guarantee covers the use of ab138386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 48 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Essential role in G1 cell cycle arrest. Phosphorylates and activates members of the AMPK-related subfamily of protein kinases. Tumor suppressor.
  • Tissue specificity

    Ubiquitously expressed. Strongest expression in testis and fetal liver.
  • Involvement in disease

    Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) [MIM:175200]. PJS is a rare hereditary disease in which there is predisposition to benign and malignant tumors of many organ systems. PJS is an autosomal dominant disorder characterized by melanocytic macules of the lips, multiple gastrointestinal hamartomatous polyps and an increased risk for various neoplasms, including gastrointestinal cancer.
    Defects in STK11 have been associated with testicular tumors (TEST) [MIM:273300]. A common solid malignancy in males. Germ cell tumors of the testis constitute 95% of all testicular neoplasms.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. LKB1 subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylated by a cAMP-dependent protein kinase.
  • Cellular localization

    Nucleus. Cytoplasm. Relocates to the cytoplasm when bound to CAB39 and STRAD or CAB39 and ALS2CR2.
  • Target information above from: UniProt accession Q15831 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6794 Human
    • Entrez Gene: 20869 Mouse
    • Omim: 602216 Human
    • SwissProt: Q15831 Human
    • SwissProt: Q9WTK7 Mouse
    • Unigene: 515005 Human
    • Alternative names

      • hLKB1 antibody
      • Liver kinase B1 antibody
      • LKB1 antibody
      • PJS antibody
      • Polarization related protein LKB1 antibody
      • Renal carcinoma antigen NY-REN-19 antibody
      • Serine/Threonine Kinase 11 antibody
      • Serine/threonine protein kinase 11 antibody
      • Serine/threonine protein kinase LKB1 antibody
      • Serine/threonine protein kinase STK11 antibody
      • Serine/threonine-protein kinase 11 antibody
      • Serine/threonine-protein kinase LKB1 antibody
      • Serine/threonine-protein kinase XEEK1 antibody
      • Stk11 antibody
      • STK11_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LKB1 (phospho S428) antibody - C-terminal (ab138386)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LKB1 (phospho S428) antibody - C-terminal (ab138386)
      Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling LKB1 (phospho S428) with ab138386 at 1/50.
    • Western blot - Anti-LKB1 (phospho S428) antibody - C-terminal (ab138386)
      Western blot - Anti-LKB1 (phospho S428) antibody - C-terminal (ab138386)
      Anti-LKB1 (phospho S428) antibody - C-terminal (ab138386) at 1/500 dilution + CV-1 cell lysate - forskolin treated at 30 µg

      Predicted band size: 48 kDa

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab138386? Please let us know so that we can cite the reference in this datasheet.

    ab138386 has been referenced in 1 publication.

    • Li F  et al. BET inhibitor JQ1 suppresses cell proliferation via inducing autophagy and activating LKB1/AMPK in bladder cancer cells. Cancer Med 8:4792-4805 (2019). PubMed: 31250978

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