Key features and details
- Rabbit polyclonal to LKB1 (phospho T189)
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-LKB1 (phospho T189) antibody
See all LKB1 primary antibodies
DescriptionRabbit polyclonal to LKB1 (phospho T189)
SpecificityDetects endogenous levels of LKB1 only when phosphorylated at threonine 189.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic phosphopeptide derived from human LKB1 around the phosphorylation site of throsine 189 (G-G-TP-L-K).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab60987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 48 kDa).|
FunctionEssential role in G1 cell cycle arrest. Phosphorylates and activates members of the AMPK-related subfamily of protein kinases. Tumor suppressor.
Tissue specificityUbiquitously expressed. Strongest expression in testis and fetal liver.
Involvement in diseaseDefects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) [MIM:175200]. PJS is a rare hereditary disease in which there is predisposition to benign and malignant tumors of many organ systems. PJS is an autosomal dominant disorder characterized by melanocytic macules of the lips, multiple gastrointestinal hamartomatous polyps and an increased risk for various neoplasms, including gastrointestinal cancer.
Defects in STK11 have been associated with testicular tumors (TEST) [MIM:273300]. A common solid malignancy in males. Germ cell tumors of the testis constitute 95% of all testicular neoplasms.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. LKB1 subfamily.
Contains 1 protein kinase domain.
modificationsPhosphorylated by a cAMP-dependent protein kinase.
Cellular localizationNucleus. Cytoplasm. Relocates to the cytoplasm when bound to CAB39 and STRAD or CAB39 and ALS2CR2.
- Information by UniProt
- hLKB1 antibody
- Liver kinase B1 antibody
- LKB1 antibody
All lanes : Anti-LKB1 (phospho T189) antibody (ab60987) at 1/500 dilution
Lane 1 : Extracts from NIH/3T3 cells,
treated with PMA (125ng/ml, 30mins).
Lane 2 : Extracts from NIH/3T3 cells,
treated with PMA (125ng/ml, 30mins) with immunizing phosphopeptide
Predicted band size: 48 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
ab60987 has not yet been referenced specifically in any publications.