Product nameAnti-LLGL1 antibody
See all LLGL1 primary antibodies
DescriptionRabbit polyclonal to LLGL1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow
- This antibody gave a positive signal in the following tissue lysates; Human brain and Mouse brain.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab39292 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 115 kDa).|
FunctionCortical cytoskeleton protein found in a complex involved in maintaining cell polarity and epithelial integrity. Involved in the regulation of mitotic spindle orientation, proliferation, differentiation and tissue organization of neuroepithelial cells.
Tissue specificityExpressed in brain, kidney, and muscle but is barely seen in heart and placenta. Down-regulated or lost in all cell lines and in most of the tumor samples analyzed. Loss was associated with advanced stage of the disease.
Sequence similaritiesBelongs to the WD repeat L(2)GL family.
Contains 14 WD repeats.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR (By similarity). Phosphorylated at least at Ser-663 by PRKCI.
Cellular localizationCytoplasm > cytoskeleton. Localized to the lateral membrane during the polarization and formation cell-cell contacts.
- Information by UniProt
- DLG4 antibody
- DLG4, formerly antibody
- Hugl 1 antibody
All lanes : Anti-LLGL1 antibody (ab39292) at 1 µg
Lane 1 : Human brain total protein lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 115 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
Additional bands at: 26 kDa, 30 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.