Overview

  • Product name
  • Description
    Rabbit polyclonal to LLGL1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Lgl1.

    Read Abcam's proprietary immunogen policy (Peptide available as ab39291.)

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates; Human brain and Mouse brain.

Properties

Applications

Our Abpromise guarantee covers the use of ab39292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 115 kDa).

Target

  • Function
    Cortical cytoskeleton protein found in a complex involved in maintaining cell polarity and epithelial integrity. Involved in the regulation of mitotic spindle orientation, proliferation, differentiation and tissue organization of neuroepithelial cells.
  • Tissue specificity
    Expressed in brain, kidney, and muscle but is barely seen in heart and placenta. Down-regulated or lost in all cell lines and in most of the tumor samples analyzed. Loss was associated with advanced stage of the disease.
  • Sequence similarities
    Belongs to the WD repeat L(2)GL family.
    Contains 14 WD repeats.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity). Phosphorylated at least at Ser-663 by PRKCI.
  • Cellular localization
    Cytoplasm > cytoskeleton. Localized to the lateral membrane during the polarization and formation cell-cell contacts.
  • Information by UniProt
  • Database links
  • Alternative names
    • DLG4 antibody
    • DLG4, formerly antibody
    • Hugl 1 antibody
    • HUGL antibody
    • Hugl-1 antibody
    • HUGL1 antibody
    • Human homolog to the D-lgl gene protein antibody
    • L2GL1_HUMAN antibody
    • Lethal giant larvae homolog 1 (Drosophila) antibody
    • Lethal giant larvae homolog 1 antibody
    • Lethal(2) giant larvae protein homolog 1 antibody
    • LLGL antibody
    • Llgl1 antibody
    • Ly 49G2 antibody
    • Ly49G2 antibody
    • Mgl1 antibody
    • Mlgl antibody
    see all

Images

  • All lanes : Anti-LLGL1 antibody (ab39292) at 1 µg

    Lane 1 : Human brain total protein lysate
    Lane 2 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size: 115 kDa
    Observed band size: 125 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 26 kDa, 30 kDa, 50 kDa. We are unsure as to the identity of these extra bands.



    Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

References

This product has been referenced in:
  • Song J  et al. Hugl-1 induces apoptosis in esophageal carcinoma cells both in vitro and in vivo. World J Gastroenterol 19:4127-36 (2013). WB ; Human . Read more (PubMed: 23864775) »
See 1 Publication for this product

Customer reviews and Q&As

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (various: incl. SW620(+) HEK293 Ms Brain)
Loading amount
30 µg
Specification
various: incl. SW620(+) HEK293 Ms Brain
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C

Mr. Antibody Solutions

Verified customer

Submitted Nov 14 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up