Key features and details
- Rabbit polyclonal to LMAN1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab32583 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/1000. Detects a band of approximately 57.5 kDa.|
FunctionMannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins.
Involvement in diseaseDefects in LMAN1 are THE cause of factor V and factor VIII combined deficiency type 1 (F5F8D1) [MIM:227300]; also known as multiple coagulation factor deficiency I (MCFD1). F5F8D1 is an autosomal recessive blood coagulation disorder characterized by bleeding symptoms similar to those in hemophilia or parahemophilia, that are caused by single deficiency of FV or FVIII, respectively. The most common symptoms are epistaxis, menorrhagia, and excessive bleeding during or after trauma. Plasma levels of coagulation factors V and VIII are in the range of 5 to 30% of normal.
Sequence similaritiesContains 1 L-type lectin-like domain.
modificationsThe N-terminal may be partly blocked.
Cellular localizationEndoplasmic reticulum-Golgi intermediate compartment membrane. Golgi apparatus membrane. Endoplasmic reticulum membrane.
- Information by UniProt
- Endoplasmic reticulum golgi intermediate compartment protein 53 antibody
- ER-Golgi intermediate compartment 53 kDa protein antibody
- ERGIC-53 antibody
ab32583 labelling LMAN1 in p19 cells by Immunocytochemistry/Immunofluorescence.
ab32583 labelling LMAN1 in HMVEC cells by Immunocytochemistry/Immunofluorescence.
ab32583 labelling LMAN1 in A549 cells by Immunocytochemistry/Immunofluorescence.
All lanes : Anti-LMAN1 antibody (ab32583) at 1/1000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat brain lysate with Immunising peptide
Observed band size: 57.5 kDa why is the actual band size different from the predicted?
ab32583 has not yet been referenced specifically in any publications.