Overview

  • Product name

    Anti-LMAN1 antibody [EPR6980]
    See all LMAN1 primary antibodies
  • Description

    Rabbit monoclonal [EPR6980] to LMAN1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human LMAN1 (P49257).

  • Positive control

    • HeLa cell lysates NCI-H460 cell lysates JAR cell lysates Papillary adenocarcinoma tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab126720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 58 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.

ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins.
    • Tissue specificity

      Ubiquitous.
    • Involvement in disease

      Defects in LMAN1 are THE cause of factor V and factor VIII combined deficiency type 1 (F5F8D1) [MIM:227300]; also known as multiple coagulation factor deficiency I (MCFD1). F5F8D1 is an autosomal recessive blood coagulation disorder characterized by bleeding symptoms similar to those in hemophilia or parahemophilia, that are caused by single deficiency of FV or FVIII, respectively. The most common symptoms are epistaxis, menorrhagia, and excessive bleeding during or after trauma. Plasma levels of coagulation factors V and VIII are in the range of 5 to 30% of normal.
    • Sequence similarities

      Contains 1 L-type lectin-like domain.
    • Post-translational
      modifications

      The N-terminal may be partly blocked.
    • Cellular localization

      Endoplasmic reticulum-Golgi intermediate compartment membrane. Golgi apparatus membrane. Endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Endoplasmic reticulum golgi intermediate compartment protein 53 antibody
      • ER-Golgi intermediate compartment 53 kDa protein antibody
      • ERGIC-53 antibody
      • ERGIC53 antibody
      • ERGIC53 like protein antibody
      • F5F8D antibody
      • FMFD1 antibody
      • Gp58 antibody
      • Intracellular mannose specific lectin antibody
      • Intracellular mannose-specific lectin MR60 antibody
      • Lectin mannose binding 1 antibody
      • Lectin mannose-binding 1 antibody
      • Lman1 antibody
      • LMAN1 like protein antibody
      • LMAN1_HUMAN antibody
      • MCFD1 antibody
      • MR60 antibody
      • Protein ERGIC-53 antibody
      see all

    Images

    • All lanes : Anti-LMAN1 antibody [EPR6980] (ab126720) at 1/1000 dilution

      Lane 1 : HeLa cell lysates
      Lane 2 : NCI-H460 cell lysates
      Lane 3 : JAR cell lysates

      Predicted band size: 58 kDa

    • ab126720, at 1/50 dilution, stains LMAN1 in paraffin-embedded papillary adenocarcinoma tissue by immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab126720 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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