Overview

  • Product name

    Anti-LMAN1 antibody [OTI1B8]
    See all LMAN1 primary antibodies
  • Description

    Mouse monoclonal [OTI1B8] to LMAN1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Rat, Dog, Human, Monkey
  • Immunogen

    Recombinant full length protein corresponding to Human LMAN1. Produced in HEK-293T cells (NP_005561).
    Database link: P49257

  • Positive control

    • WB: HEK-293T cell lysate transfected with pCMV6-ENTRY LMAN1 cDNA; A549, COS-7, HeLa, HepG2, HT-29, Jurkat, MCF7, MDCK and PC-12 cell extracts; Wild-type HEK-293T cell lysate.
  • General notes

    Clone OTI1B8 (formerly 1B8).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    Purified from cell culture supernatant by affinity chromatography..
  • Clonality

    Monoclonal
  • Clone number

    OTI1B8
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab118407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/500. Predicted molecular weight: 58 kDa.

Target

  • Function

    Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins.
  • Tissue specificity

    Ubiquitous.
  • Involvement in disease

    Defects in LMAN1 are THE cause of factor V and factor VIII combined deficiency type 1 (F5F8D1) [MIM:227300]; also known as multiple coagulation factor deficiency I (MCFD1). F5F8D1 is an autosomal recessive blood coagulation disorder characterized by bleeding symptoms similar to those in hemophilia or parahemophilia, that are caused by single deficiency of FV or FVIII, respectively. The most common symptoms are epistaxis, menorrhagia, and excessive bleeding during or after trauma. Plasma levels of coagulation factors V and VIII are in the range of 5 to 30% of normal.
  • Sequence similarities

    Contains 1 L-type lectin-like domain.
  • Post-translational
    modifications

    The N-terminal may be partly blocked.
  • Cellular localization

    Endoplasmic reticulum-Golgi intermediate compartment membrane. Golgi apparatus membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Endoplasmic reticulum golgi intermediate compartment protein 53 antibody
    • ER-Golgi intermediate compartment 53 kDa protein antibody
    • ERGIC-53 antibody
    • ERGIC53 antibody
    • ERGIC53 like protein antibody
    • F5F8D antibody
    • FMFD1 antibody
    • Gp58 antibody
    • Intracellular mannose specific lectin antibody
    • Intracellular mannose-specific lectin MR60 antibody
    • Lectin mannose binding 1 antibody
    • Lectin mannose-binding 1 antibody
    • Lman1 antibody
    • LMAN1 like protein antibody
    • LMAN1_HUMAN antibody
    • MCFD1 antibody
    • MR60 antibody
    • Protein ERGIC-53 antibody
    see all

Images

  • All lanes : Anti-LMAN1 antibody [OTI1B8] (ab118407) at 1/200 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY LMAN1 cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 58 kDa

  • All lanes : Anti-LMAN1 antibody [OTI1B8] (ab118407) at 1/200 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell extract
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
    Lane 3 : HT29 (human colorectal adenocarcinoma cell line) cell extract
    Lane 4 : A549 (human lung carcinoma cell line) cell extract
    Lane 5 : COS-7 (african green monkey kidney fibroblast-like cell line) cell extract
    Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell extract
    Lane 7 : MDCK (canine kidney cell line) cell extract
    Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell extract
    Lane 9 : MCF7 (human breast adenocarcinoma cell line) cell extract

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 58 kDa

  • Lanes 1-2 : Anti-LMAN1 antibody [OTI1B8] (ab118407) at 1/1000 dilution
    Lanes 3-4 : anti-HSP90AB1

    Lanes 1 & 3 : Wild-type HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lanes 2 & 4 : LMAN1-knockout HEK-293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 58 kDa

References

ab118407 has not yet been referenced specifically in any publications.

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