Anti-LMCD1 antibody (ab121788)
Key features and details
- Rabbit polyclonal to LMCD1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LMCD1 antibody
See all LMCD1 primary antibodies -
Description
Rabbit polyclonal to LMCD1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence, corresponding to amino acids 160-293 of Human LMCD1.
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Positive control
- Human cerebellum tissue; RT-4, U-251 MG cell lysate; Human Liver and Tonsil lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121788 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Transcriptional cofactor that restricts GATA6 function by inhibiting DNA-binding, resulting in repression of GATA6 transcriptional activation of downstream target genes. Represses GATA6-mediated trans activation of lung- and cardiac tissue-specific promoters. Inhibits DNA-binding by GATA4 and GATA1 to the cTNC promoter (By similarity). Plays a critical role in the development of cardiac hypertrophy via activation of calcineurin/nuclear factor of activated T cells signaling pathway. -
Tissue specificity
Expressed in the heart (at protein level). Expressed in many tissues with highest abundance in skeletal muscle. -
Sequence similarities
Contains 2 LIM zinc-binding domains.
Contains 1 PET domain. -
Domain
The LIM zinc-binding domains and the Cys-rich region mediate interaction with GATA6. -
Cellular localization
Cytoplasm. Nucleus. May shuttle between the cytoplasm and the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 29995 Human
- Omim: 604859 Human
- SwissProt: Q9NZU5 Human
- Unigene: 475353 Human
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Alternative names
- Dyxin antibody
- LIM and cysteine rich domains 1 antibody
- LIM and cysteine rich domains protein 1 antibody
see all
Images
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All lanes : Anti-LMCD1 antibody (ab121788) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique. -
Immunofluorescent staining of Human cell line A-431 shows positivity in plasma membrane and cell junctions. Recommended concentration of ab121788 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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ab121788, at 1/400 dilution, staining LMCD1 in paraffin-embedded Human cerebellum tissue, by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab121788 has not yet been referenced specifically in any publications.