Product nameAnti-LMCD1 antibody
See all LMCD1 primary antibodies
DescriptionRabbit polyclonal to LMCD1
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
antigen sequence, corresponding to amino acids 160-293 of Human LMCD1.
- Human cerebellum tissue; RT-4, U-251 MG cell lysate; Human Liver and Tonsil lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121788 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|WB||1/250 - 1/500.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionTranscriptional cofactor that restricts GATA6 function by inhibiting DNA-binding, resulting in repression of GATA6 transcriptional activation of downstream target genes. Represses GATA6-mediated trans activation of lung- and cardiac tissue-specific promoters. Inhibits DNA-binding by GATA4 and GATA1 to the cTNC promoter (By similarity). Plays a critical role in the development of cardiac hypertrophy via activation of calcineurin/nuclear factor of activated T cells signaling pathway.
Tissue specificityExpressed in the heart (at protein level). Expressed in many tissues with highest abundance in skeletal muscle.
Sequence similaritiesContains 2 LIM zinc-binding domains.
Contains 1 PET domain.
DomainThe LIM zinc-binding domains and the Cys-rich region mediate interaction with GATA6.
Cellular localizationCytoplasm. Nucleus. May shuttle between the cytoplasm and the nucleus.
- Information by UniProt
- Dyxin antibody
- LIM and cysteine rich domains 1 antibody
- LIM and cysteine rich domains protein 1 antibody
All lanes : Anti-LMCD1 antibody (ab121788) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique.
Immunofluorescent staining of Human cell line A-431 shows positivity in plasma membrane and cell junctions. Recommended concentration of ab121788 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121788, at 1/400 dilution, staining LMCD1 in paraffin-embedded Human cerebellum tissue, by Immunohistochemistry.
ab121788 has not yet been referenced specifically in any publications.