Product nameAnti-LMCD1 antibody [EPR11443(B)]
See all LMCD1 primary antibodies
DescriptionRabbit monoclonal [EPR11443(B)] to LMCD1
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide within Human LMCD1. The exact sequence is proprietary.
Database link: Q9NZU5
- Human skeletal muscle and SH-SY5Y lysates.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA disodium salt, 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab169760 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).|
|IP||1/10 - 1/100.|
FunctionTranscriptional cofactor that restricts GATA6 function by inhibiting DNA-binding, resulting in repression of GATA6 transcriptional activation of downstream target genes. Represses GATA6-mediated trans activation of lung- and cardiac tissue-specific promoters. Inhibits DNA-binding by GATA4 and GATA1 to the cTNC promoter (By similarity). Plays a critical role in the development of cardiac hypertrophy via activation of calcineurin/nuclear factor of activated T cells signaling pathway.
Tissue specificityExpressed in the heart (at protein level). Expressed in many tissues with highest abundance in skeletal muscle.
Sequence similaritiesContains 2 LIM zinc-binding domains.
Contains 1 PET domain.
DomainThe LIM zinc-binding domains and the Cys-rich region mediate interaction with GATA6.
Cellular localizationCytoplasm. Nucleus. May shuttle between the cytoplasm and the nucleus.
- Information by UniProt
- Dyxin antibody
- LIM and cysteine rich domains 1 antibody
- LIM and cysteine rich domains protein 1 antibody
Detection of LMCD1 by Western Blot of Immunprecipitate.
Human skeletal muscle lysate immunoprecipitated using ab169760 at 1/10 dilution (lane 1) or a 1 X PBS negative control (lane 2) and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
All lanes : Anti-LMCD1 antibody [EPR11443(B)] (ab169760) at 1/1000 dilution
Lane 1 : Human skeletal muscle lysate
Lane 2 : SH-SY5Y lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 41 kDa
ab169760 has not yet been referenced specifically in any publications.