Recombinant
RabMAb

Anti-LMCD1 antibody [EPR11444] (ab179454)

Overview

  • Product name
    Anti-LMCD1 antibody [EPR11444]
    See all LMCD1 primary antibodies
  • Description
    Rabbit monoclonal [EPR11444] to LMCD1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human LMCD1 aa 200-300 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9NZU5

  • Positive control
    • Human skeletal muscle lysate; MCF7 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab179454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 41 kDa.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Transcriptional cofactor that restricts GATA6 function by inhibiting DNA-binding, resulting in repression of GATA6 transcriptional activation of downstream target genes. Represses GATA6-mediated trans activation of lung- and cardiac tissue-specific promoters. Inhibits DNA-binding by GATA4 and GATA1 to the cTNC promoter (By similarity). Plays a critical role in the development of cardiac hypertrophy via activation of calcineurin/nuclear factor of activated T cells signaling pathway.
    • Tissue specificity
      Expressed in the heart (at protein level). Expressed in many tissues with highest abundance in skeletal muscle.
    • Sequence similarities
      Contains 2 LIM zinc-binding domains.
      Contains 1 PET domain.
    • Domain
      The LIM zinc-binding domains and the Cys-rich region mediate interaction with GATA6.
    • Cellular localization
      Cytoplasm. Nucleus. May shuttle between the cytoplasm and the nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Dyxin antibody
      • LIM and cysteine rich domains 1 antibody
      • LIM and cysteine rich domains protein 1 antibody
      • LIM and cysteine-rich domains protein 1 antibody
      • LMCD1 antibody
      • LMCD1_HUMAN antibody
      see all

    Images

    • Immunofluorescent analysis of MCF7 cells labeling LMCD1 with ab179454 at 1/100 dilution.

    • Anti-LMCD1 antibody [EPR11444] (ab179454) at 1/1000 dilution + Human skeletal muscle lysate at 10 µg

      Predicted band size: 41 kDa

    • Western blot analysis on immunoprecipitation pellet from (1) Human skeletal muscle lysate or (2) 1XPBS (negative control) using ab179454, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    References

    ab179454 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab179454.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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