Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11444] to LMCD1
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human
Product nameAnti-LMCD1 antibody [EPR11444]
See all LMCD1 primary antibodies
DescriptionRabbit monoclonal [EPR11444] to LMCD1
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Human
Synthetic peptide within Human LMCD1 aa 200-300 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9NZU5
- WB: Human skeletal muscle lysate. ICC/IF: MCF7 cells. IP: LMCD1 IP in human skeletal muscle lysate.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Constituents: 0.35% Sodium citrate, 0.17% Sodium chloride, 0.03% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab179454 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 41 kDa.|
|ICC/IF||1/100 - 1/250.|
|IP||1/10 - 1/100.|
FunctionTranscriptional cofactor that restricts GATA6 function by inhibiting DNA-binding, resulting in repression of GATA6 transcriptional activation of downstream target genes. Represses GATA6-mediated trans activation of lung- and cardiac tissue-specific promoters. Inhibits DNA-binding by GATA4 and GATA1 to the cTNC promoter (By similarity). Plays a critical role in the development of cardiac hypertrophy via activation of calcineurin/nuclear factor of activated T cells signaling pathway.
Tissue specificityExpressed in the heart (at protein level). Expressed in many tissues with highest abundance in skeletal muscle.
Sequence similaritiesContains 2 LIM zinc-binding domains.
Contains 1 PET domain.
DomainThe LIM zinc-binding domains and the Cys-rich region mediate interaction with GATA6.
Cellular localizationCytoplasm. Nucleus. May shuttle between the cytoplasm and the nucleus.
- Information by UniProt
- Dyxin antibody
- LIM and cysteine rich domains 1 antibody
- LIM and cysteine rich domains protein 1 antibody
Anti-LMCD1 antibody [EPR11444] (ab179454) at 1/1000 dilution + Human skeletal muscle lysate at 10 µg
Predicted band size: 41 kDa
Immunofluorescent analysis of MCF7 (Human breast adenocarcinoma cell line) cells labeling LMCD1 with ab179454 at 1/100 dilution.
Western blot analysis on immunoprecipitation pellet from (Lane 1) Human skeletal muscle lysate or (Lane 2) 1X PBS (negative control) using ab179454, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
ab179454 has been referenced in 2 publications.
- Govatati S et al. NFATc1-E2F1-LMCD1-Mediated IL-33 Expression by Thrombin Is Required for Injury-Induced Neointima Formation. Arterioscler Thromb Vasc Biol 39:1212-1226 (2019). PubMed: 31043075
- Janjanam J et al. LIM and cysteine-rich domains 1 is required for thrombin-induced smooth muscle cell proliferation and promotes atherogenesis. J Biol Chem 293:3088-3103 (2018). PubMed: 29326163