Overview

  • Product name

    Anti-LMCD1 antibody [EPR11444] - BSA and Azide free
    See all LMCD1 primary antibodies
  • Description

    Rabbit monoclonal [EPR11444] to LMCD1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IP, WBmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human LMCD1 aa 200-300 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9NZU5

  • Positive control

    • ICC/IF: MCF7 cells. IP: LMCD1 IP in human skeletal muscle lysate.
  • General notes

    ab242401 is the carrier-free version of ab179454 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab242401 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab242401 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Transcriptional cofactor that restricts GATA6 function by inhibiting DNA-binding, resulting in repression of GATA6 transcriptional activation of downstream target genes. Represses GATA6-mediated trans activation of lung- and cardiac tissue-specific promoters. Inhibits DNA-binding by GATA4 and GATA1 to the cTNC promoter (By similarity). Plays a critical role in the development of cardiac hypertrophy via activation of calcineurin/nuclear factor of activated T cells signaling pathway.
    • Tissue specificity

      Expressed in the heart (at protein level). Expressed in many tissues with highest abundance in skeletal muscle.
    • Sequence similarities

      Contains 2 LIM zinc-binding domains.
      Contains 1 PET domain.
    • Domain

      The LIM zinc-binding domains and the Cys-rich region mediate interaction with GATA6.
    • Cellular localization

      Cytoplasm. Nucleus. May shuttle between the cytoplasm and the nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Dyxin antibody
      • LIM and cysteine rich domains 1 antibody
      • LIM and cysteine rich domains protein 1 antibody
      • LIM and cysteine-rich domains protein 1 antibody
      • LMCD1 antibody
      • LMCD1_HUMAN antibody
      see all

    Images

    • Immunofluorescent analysis of MCF7 (Human breast adenocarcinoma cell line) cells labeling LMCD1 with ab179454 at 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing sodium citrate, sodium chloride, EDTA, PBS, BSA and glycerol (ab179454)

    • Western blot analysis on immunoprecipitation pellet from (Lane 1) Human skeletal muscle lysate or (Lane 2) 1X PBS (negative control) using ab179454, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

      This data was developed using the same antibody clone in a different buffer formulation containing sodium citrate, sodium chloride, EDTA, PBS, BSA and glycerol (ab179454)

    References

    ab242401 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab242401.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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