Recombinant Anti-LMCD1 antibody [EPR11444] - BSA and Azide free (ab242401)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11444] to LMCD1 - BSA and Azide free
- Suitable for: ICC/IF, IP, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-LMCD1 antibody [EPR11444] - BSA and Azide free
See all LMCD1 primary antibodies -
Description
Rabbit monoclonal [EPR11444] to LMCD1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: MCF7 cells. IP: LMCD1 IP in human skeletal muscle lysate.
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General notes
ab242401 is the carrier-free version of ab179454.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11444 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab242401 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 41 kDa. |
Target
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Function
Transcriptional cofactor that restricts GATA6 function by inhibiting DNA-binding, resulting in repression of GATA6 transcriptional activation of downstream target genes. Represses GATA6-mediated trans activation of lung- and cardiac tissue-specific promoters. Inhibits DNA-binding by GATA4 and GATA1 to the cTNC promoter (By similarity). Plays a critical role in the development of cardiac hypertrophy via activation of calcineurin/nuclear factor of activated T cells signaling pathway. -
Tissue specificity
Expressed in the heart (at protein level). Expressed in many tissues with highest abundance in skeletal muscle. -
Sequence similarities
Contains 2 LIM zinc-binding domains.
Contains 1 PET domain. -
Domain
The LIM zinc-binding domains and the Cys-rich region mediate interaction with GATA6. -
Cellular localization
Cytoplasm. Nucleus. May shuttle between the cytoplasm and the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 29995 Human
- Omim: 604859 Human
- SwissProt: Q9NZU5 Human
- Unigene: 475353 Human
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Alternative names
- Dyxin antibody
- LIM and cysteine rich domains 1 antibody
- LIM and cysteine rich domains protein 1 antibody
see all
Images
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Western blot analysis on immunoprecipitation pellet from (Lane 1) Human skeletal muscle lysate or (Lane 2) 1X PBS (negative control) using ab179454, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
This data was developed using the same antibody clone in a different buffer formulation containing sodium citrate, sodium chloride, EDTA, PBS, BSA and glycerol (ab179454)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab242401 has not yet been referenced specifically in any publications.