ChIP abreview for Anti-LMO2 antibody

Poor
Abreviews
Application
ChIP
Sample
Mouse Cell lysate - nuclear (haematopoietic progenitor cell line HPC-7)
Specification
haematopoietic progenitor cell line HPC-7
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: both 0.4% and 1% formaldehyde
Detection step
Real-time PCR
Positive control
i) a competitor antibody known to work
ii) a regulatory region of a gene known to bind this transcription factor
Negative control
a regulatory region of a gene known to be an open region of chromatin but devoid of this transcription factor

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C

Additional data

Abcam response
This antibody has not been previously tested in mouse or in ChIP. It is therefore difficult to judge whether this antibody is not suitable for use in mouse, or in ChIP, or both. We would welcome more feedback from our customers about how useful this antibody is in ChIP, or in detecting the mouse LMO2 protein.

Abcam user community

Verified customer

Submitted Oct 06 2009

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