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    lmo2-antibody-ab219995.pdf

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Anti-LMO2 antibody (ab219995)

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Immunocytochemistry/ Immunofluorescence - Anti-LMO2 antibody (ab219995)

    Key features and details

    • Rabbit polyclonal to LMO2
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human LMO2 protein (ab152503)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-LMO2 antibody
      See all LMO2 primary antibodies
    • Description

      Rabbit polyclonal to LMO2
    • Host species

      Rabbit
    • Tested applications

      Suitable for: ICC/IFmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis
    • Immunogen

      Recombinant fragment corresponding to Human LMO2 aa 8-157.
      Sequence:

      KSLDPSEEPVDEVLQIPPSLLTCGGCQQNIGDRYFLKAIDQYWHEDCLSC DLCGCRLGEVGRRLYYKLGRKLCRRDYLRLFGQDGLCASCDKRIRAYEMT MRVKDKVYHLECFKCAACQKHFCVGDRYLLINSDIVCEQDIYEWTKINGM


      Database link: P25791
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • REH cells.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.20
      Preservative: 0.02% Sodium azide
      Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cardiovascular
      • Blood
      • Other

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human LMO2 protein (ab152503)

    Applications

    Our Abpromise guarantee covers the use of ab219995 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF Use a concentration of 0.25 - 2 µg/ml.

    Target

    • Function

      Acts with TAL1/SCL to regulate red blood cell development. Also acts with LDB1 to maintain erythroid precursors in an immature state.
    • Involvement in disease

      Note=A chromosomal aberration involving LMO2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(11,14)(p13;q11) with TCRD.
    • Sequence similarities

      Contains 2 LIM zinc-binding domains.
    • Domain

      The second LIM zinc-binding domain interacts with KDM5A.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession P25791 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 614876 Cow
      • Entrez Gene: 4005 Human
      • Entrez Gene: 16909 Mouse
      • Entrez Gene: 108713784 Xenopus laevis
      • Entrez Gene: 394388 Xenopus laevis
      • Entrez Gene: 496679 Xenopus tropicalis
      • Entrez Gene: 30332 Zebrafish
      • Omim: 180385 Human
      • SwissProt: Q1LZ94 Cow
      • SwissProt: P25791 Human
      • SwissProt: P25801 Mouse
      • SwissProt: Q90XH3 Xenopus laevis
      • SwissProt: Q9PTJ3 Zebrafish
      • Unigene: 34560 Human
      • Unigene: 29266 Mouse
      • Unigene: 9549 Xenopus laevis
      • Unigene: 79808 Zebrafish
      see all
    • Alternative names

      • Cysteine rich protein TTG 2 antibody
      • Cysteine rich protein TTG2 antibody
      • Cysteine-rich protein TTG-2 antibody
      • LIM domain only 2 (rhombotin like 1) antibody
      • LIM domain only 2 antibody
      • LIM domain only protein 2 antibody
      • LMO 2 antibody
      • LMO-2 antibody
      • lmo2 antibody
      • RBTN 2 antibody
      • RBTN L1 antibody
      • RBTN2 antibody
      • RBTN2_HUMAN antibody
      • RBTNL 1 antibody
      • RBTNL1 antibody
      • RHOM 2 antibody
      • RHOM2 antibody
      • Rhombotin 2 antibody
      • Rhombotin like 1 antibody
      • Rhombotin-2 antibody
      • Rhombotin2 antibody
      • T cell translocation gene 2 antibody
      • T cell translocation protein 2 antibody
      • T-cell translocation protein 2 antibody
      • TTG 2 antibody
      • TTG2 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-LMO2 antibody (ab219995)
      Immunocytochemistry/ Immunofluorescence - Anti-LMO2 antibody (ab219995)

      Immunofluorescent analysis of REH cells (PFA-fixed/Triton X-100 permeabilized) labeling LMO2 with ab219995 at 4 µg/mL (green).

    Protocols

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab219995? Please let us know so that we can cite the reference in this datasheet.

    ab219995 has not yet been referenced specifically in any publications.

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