Synthetic peptide within Human LMO2. The exact sequence is proprietary.
WB: Raji and HEK-293 cell lysate and Fetal kidney, mouse E14 embryo, and rat E14 embryo tissue lysate
IP: Raji whole cell lysate
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
ab91652 (purified) at 1:30 dilution (2µg) immunoprecipitating LMO2 in Raji whole cell lysate. Lane 1 (input): Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10µg Lane 2 (+): ab91652 & Raji whole cell lysate Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab91652 in Raji whole cell lysate For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1:1000 dilution. Blocking and diluting buffer: 5% NFDM/TBST.
Western blot - Anti-LMO2 antibody [EP3257] (ab91652)
All lanes : Anti-LMO2 antibody [EP3257] (ab91652) at 1/1000 dilution (Unpurified)
Lane 1 : Raji cell lysate Lane 2 : Fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution