Overview

  • Product name

    Anti-LMO2 antibody [EP3257]
    See all LMO2 primary antibodies
  • Description

    Rabbit monoclonal [EP3257] to LMO2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human LMO2. The exact sequence is proprietary.

  • Positive control

    • WB: Raji and HEK-293 cell lysate and Fetal kidney, mouse E14 embryo, and rat E14 embryo tissue lysate IP: Raji whole cell lysate
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP3257
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab91652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 18 kDa.
IP 1/30.

For unpurified use at 1/100 - 1/500

Target

  • Function

    Acts with TAL1/SCL to regulate red blood cell development. Also acts with LDB1 to maintain erythroid precursors in an immature state.
  • Involvement in disease

    Note=A chromosomal aberration involving LMO2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(11,14)(p13;q11) with TCRD.
  • Sequence similarities

    Contains 2 LIM zinc-binding domains.
  • Domain

    The second LIM zinc-binding domain interacts with KDM5A.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cysteine rich protein TTG 2 antibody
    • Cysteine rich protein TTG2 antibody
    • Cysteine-rich protein TTG-2 antibody
    • LIM domain only 2 (rhombotin like 1) antibody
    • LIM domain only 2 antibody
    • LIM domain only protein 2 antibody
    • LMO 2 antibody
    • LMO-2 antibody
    • lmo2 antibody
    • RBTN 2 antibody
    • RBTN L1 antibody
    • RBTN2 antibody
    • RBTN2_HUMAN antibody
    • RBTNL 1 antibody
    • RBTNL1 antibody
    • RHOM 2 antibody
    • RHOM2 antibody
    • Rhombotin 2 antibody
    • Rhombotin like 1 antibody
    • Rhombotin-2 antibody
    • Rhombotin2 antibody
    • T cell translocation gene 2 antibody
    • T cell translocation protein 2 antibody
    • T-cell translocation protein 2 antibody
    • TTG 2 antibody
    • TTG2 antibody
    see all

Images

  • ab91652 (purified) at 1:30 dilution (2µg) immunoprecipitating LMO2 in Raji whole cell lysate.
    Lane 1 (input): Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10µg
    Lane 2 (+): ab91652 & Raji whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab91652 in Raji whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • All lanes : Anti-LMO2 antibody [EP3257] (ab91652) at 0.3 µg/ml (purified)

    Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates
    Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
    Lane 3 : Mouse E14 embryo lysates
    Lane 4 : Rat E14 embryo lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 18 kDa



    Blocking and diluting buffer: 5% NFDM/TBST. 

  • All lanes : Anti-LMO2 antibody [EP3257] (ab91652) at 1/1000 dilution (Unpurified)

    Lane 1 : Raji cell
    lysate
    Lane 2 : Fetal kidney
    lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 18 kDa

References

This product has been referenced in:

  • Bergiers I  et al. Single-cell transcriptomics reveals a new dynamical function of transcription factors during embryonic hematopoiesis. Elife 7:N/A (2018). Read more (PubMed: 29555020) »
  • Liu Y  et al. LMO2 Enhances Lamellipodia/Filopodia Formation in Basal-Type Breast Cancer Cells by Mediating ARP3-Profilin1 Interaction. Med Sci Monit 23:695-703 (2017). Read more (PubMed: 28170369) »
See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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