Anti-LMO4 antibody (ab229226)
Key features and details
- Rabbit polyclonal to LMO4
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-LMO4 antibody
See all LMO4 primary antibodies -
Description
Rabbit polyclonal to LMO4 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow -
Immunogen
Recombinant full length protein corresponding to Human LMO4 aa 1 to the C-terminus.
Database link: P61968 -
Positive control
- WB: LMO4-transfected HEK-293T whole cell extract. IHC-P: Human breast carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229226 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/1000.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 18 kDa.
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Notes |
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IHC-P
1/100 - 1/1000. |
WB
1/1000 - 1/10000. Predicted molecular weight: 18 kDa. |
Target
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Function
Probable transcriptional factor. -
Sequence similarities
Contains 2 LIM zinc-binding domains. - Information by UniProt
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Database links
- Entrez Gene: 614212 Cow
- Entrez Gene: 8543 Human
- Entrez Gene: 16911 Mouse
- Omim: 603129 Human
- SwissProt: Q3SWZ8 Cow
- SwissProt: P61968 Human
- SwissProt: P61969 Mouse
- Unigene: 436792 Human
see all -
Alternative names
- Breast tumor autoantigen antibody
- LIM domain only 4 antibody
- LIM domain only protein 4 antibody
see all
Images
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All lanes : Anti-LMO4 antibody (ab229226) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : LMO4-transfected HEK-293T whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 18 kDa12% SDS-PAGE gel.
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Paraffin-embedded human breast carcinoma tissue stained for LMO4 using ab229226 at 1/500 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab229226 has been referenced in 3 publications.
- Authiat MM et al. Preferential Involvement of BRCA1/BARD1, Not Tip60/Fe65, in DNA Double-Strand Break Repair in Presenilin-1 P117L Alzheimer Models. Neural Plast 2022:3172861 (2022). PubMed: 35237315
- Ma X et al. FOXM1 Promotes Head and Neck Squamous Cell Carcinoma via Activation of the Linc-ROR/LMO4/AKT/PI3K Axis. Front Oncol 11:658712 (2021). PubMed: 34447693
- Liu L et al. Circ_0058124 Aggravates the Progression of Papillary Thyroid Carcinoma by Activating LMO4 Expression via Targeting miR-370-3p. Cancer Manag Res 12:9459-9470 (2020). PubMed: 33061633