Key features and details
- Rabbit polyclonal to LMO7
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LMO7 antibody
See all LMO7 primary antibodies
DescriptionRabbit polyclonal to LMO7
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
- WB: HeLa whole cell lysate (ab150035). ICC/IF: A431 cells. IHC-P: Human duodenum, skeletal muscle, testis and uterine cervix tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224113 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 154,158,185,191,193 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
Tissue specificityWidely expressed. Isoform 2 and isoform 4 are predominantly expressed in brain.
Sequence similaritiesContains 1 CH (calponin-homology) domain.
Contains 1 LIM zinc-binding domain.
Contains 1 PDZ (DHR) domain.
- Information by UniProt
- F box only protein 20 antibody
- F box protein Fbx20 antibody
- F-box only protein 20 antibody
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for LMO7 (green) using ab224113 at 4 µg/ml in ICC/IF.
Lane 1 : Anti-LMO7 antibody (ab224113) at 0.4 µg/ml (HeLa cell lysate)
Lane 2 : Anti-LMO7 antibody (ab224113) at 0.4 µg/ml (HEK-293 lysate)
All lanes :
Predicted band size: 154,158,185,191,193 kDa
Western blot analysis of HeLa cell lysate using ab224113 at 0.4 µg/ml.
Paraffin-embedded human duodenum tissue stained for LMO7 using ab224113 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human skeletal muscle tissue stained for LMO7 using ab224113 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human testis tissue stained for LMO7 using ab224113 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human uterine cervix tissue stained for LMO7 using ab224113 at 1/200 dilution in immunohistochemical analysis.
ab224113 has not yet been referenced specifically in any publications.