• Product name

    Anti-LMW Kininogen antibody
  • Description

    Rabbit polyclonal to LMW Kininogen
  • Host species

  • Tested applications

    Suitable for: ICC/IF, WB, IP, ELISA, RIA, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Human Kininogen (LMW) purified from human plasma

  • Positive control

    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal liver.



Our Abpromise guarantee covers the use of ab79650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 20 µg/ml. Predicted molecular weight: 48 kDa.
IP Use at an assay dependent concentration.
ELISA Use a concentration of 4 - 10 µg/ml.
RIA Use a concentration of 4 - 10 µg/ml.
IHC-P Use a concentration of 5 µg/ml.


  • Function

    (1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.
  • Tissue specificity

    Secreted in plasma. T-kinin is detected in malignant ovarian, colon and breast carcinomas, but not in benign tumors.
  • Involvement in disease

    Defects in KNG1 are the cause of high molecular weight kininogen deficiency (HMWK deficiency) [MIM:228960]. HMWK deficiency is an autosomal recessive coagulation defect. Patients with HWMK deficiency do not have a hemorrhagic tendency, but they exhibit abnormal surface-mediated activation of fibrinolysis.
  • Sequence similarities

    Contains 3 cystatin kininogen-type domains.
  • Post-translational

    Bradykinin is released from kininogen by plasma kallikrein.
    Hydroxylation of Pro-383 occurs prior to the release of bradykinin.
    Phosphorylation sites are present in the extracellular medium.
    N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
  • Cellular localization

    Secreted > extracellular space.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alpha-2-thiol proteinase inhibitor antibody
    • BDK antibody
    • BK antibody
    • Fitzgerald factor antibody
    • High molecular weight kininogen antibody
    • HMWK antibody
    • Ile-Ser-Bradykinin antibody
    • Kallidin I antibody
    • Kallidin II antibody
    • Kininogen 1 antibody
    • KNG antibody
    • KNG1 antibody
    • KNG1_HUMAN antibody
    • LMW antibody
    • Low molecular weight growth-promoting factor antibody
    • Low molecular weight kininogen antibody
    • Williams-Fitzgerald-Flaujeac factor antibody
    see all


  • ICC/IF image of ab79650 stained HepG2 cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79650, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of LMW Kininogen staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79650, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


This product has been referenced in:

  • Liu J  et al. An investigational RNAi therapeutic targeting Factor XII (ALN-F12) for the treatment of hereditary angioedema. RNA 25:255-263 (2019). Read more (PubMed: 30463937) »
  • Wang M  et al. Changes in the Glycosylation of Kininogen and the Development of a Kininogen-Based Algorithm for the Early Detection of HCC. Cancer Epidemiol Biomarkers Prev 26:795-803 (2017). Read more (PubMed: 28223431) »
See all 3 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Western blot
Human Cell lysate - whole cell (Healthy and CLL B-cells)
Loading amount
40 µg
Healthy and CLL B-cells
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Dr. Elena Kashuba

Verified customer

Submitted Apr 10 2013


I can confirm that this particular antibody is sourced and tested externally. They have done IP with cell cultures over expressing the LMW Kininogen protein. Therefore, I am sorry they do not have any data to show how it perform in the other samples.

The protocol used was apparently very similar to the one we have on the Abcam website. I hope this will be helpful to you. Please note this is a guideline only and may require some further individual optimization.


As discussed on the telephone, the amount of protein you have at the end of the IP and its purity will very much depend on the sample you are using, the expression level in the sample, as well as how much sample is loaded. This is therefore a difficult question to answer as it will be very individual. I can recommend to check the IP sample on a coomassie stained gel and WB afterward purification.

I am sorry there is no more detailed information to provide on this occasion, however I hope it will be helpful.

Read More


Please check flow cytometry protocol on our website https://www.abcam.com/index.html?pageconfig=resource&rid=11380 The ab79650 is not a labelled antibody so the following procedure will better fit https://www.abcam.com/index.html?pageconfig=resource&rid=11381 Please find attached the flow cystometry detailed guide; https://docs.abcam.com/pdf/protocols/Introduction_to_flow_cytometry_May_10.pdf Please use the search to check the conjugated secondary antibodies available in catalogue https://www.abcam.com/index.html?pageconfig=productmap&cl=918 e.g. ab97050, ab97199, ab98502, ab97068, ab97063 etc.  https://www.abcam.com/index.html?pageconfig=searchresults For ELISA please check the following links: https://www.abcam.com/index.html?pageconfig=resource&rid=11421 https://www.abcam.com/index.html? pageconfig=resource&rid=11389 CONJUGATION KITS When you choose the conjugate in flow cytometry e.g. FITC, AP, PI then you can actually decide which kit will be most suitable to conjugate the un-conjugated primary antibody? The Kits we have are EASY LINK ANTIBODY CONJUGATION KITS that can be found by clicking the following link;  https://www.abcam.com/index.html?pageconfig=resource&rid=13148

Read More
Western blot
Human Cell lysate - whole cell (Daudi cells, lung cancer A549)
Loading amount
20 µg
Daudi cells, lung cancer A549
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Blocking step
Milk as blocking agent for 5 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

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Verified customer

Submitted Aug 19 2010

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