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Customer kindly asked - the flow cuytometry protocol - The secondary antibody they can use in flow - Concentration of the antibody - Kit for labeling primary antibody with conjugates.
Asked on Nov 04 2011
Please check flow cytometry protocol on our website https://www.abcam.com/index.html?pageconfig=resource&rid=11380 The ab79650 is not a labelled antibody so the following procedure will better fit https://www.abcam.com/index.html?pageconfig=resource&rid=11381 Please find attached the flow cystometry detailed guide; https://docs.abcam.com/pdf/protocols/Introduction_to_flow_cytometry_May_10.pdf Please use the search to check the conjugated secondary antibodies available in catalogue https://www.abcam.com/index.html?pageconfig=productmap&cl=918 e.g. ab97050, ab97199, ab98502, ab97068, ab97063 etc. https://www.abcam.com/index.html?pageconfig=searchresults For ELISA please check the following links: https://www.abcam.com/index.html?pageconfig=resource&rid=11421 https://www.abcam.com/index.html? pageconfig=resource&rid=11389 CONJUGATION KITS When you choose the conjugate in flow cytometry e.g. FITC, AP, PI then you can actually decide which kit will be most suitable to conjugate the un-conjugated primary antibody? The Kits we have are EASY LINK ANTIBODY CONJUGATION KITS that can be found by clicking the following link; https://www.abcam.com/index.html?pageconfig=resource&rid=13148
Answered on Nov 04 2011