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Please provide the IP protocol for ab79650
How pure will the protein be after IP, and how much protein will we get?
Asked on Jun 08 2012
I can confirm that this particular antibody is sourced and tested externally. They have done IP with cell cultures over expressing the LMW Kininogen protein. Therefore, I am sorry they do not have any data to show how it perform in the other samples.
The protocol used was apparently very similar to the one we have on the Abcam website. I hope this will be helpful to you. Please note this is a guideline only and may require some further individual optimization.
As discussed on the telephone, the amount of protein you have at the end of the IP and its purity will very much depend on the sample you are using, the expression level in the sample, as well as how much sample is loaded. This is therefore a difficult question to answer as it will be very individual. I can recommend to check the IP sample on a coomassie stained gel and WB afterward purification.
I am sorry there is no more detailed information to provide on this occasion, however I hope it will be helpful.
Answered on Jun 08 2012