Key features and details
- Rabbit polyclonal to LMX1A
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Concentration information loading...
Purification notesCrude serum, final bleed.
Our Abpromise guarantee covers the use of ab82833 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
A minimum titer of 1/10000 is determined for one of the three peptides.
WB: 1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionActs as a transcriptional activator by binding to an A/T-rich sequence, the FLAT element, in the insulin gene promoter. Required for development of the roof plate and, in turn, for specification of dorsal cell fates in the CNS and developing vertebrae.
Tissue specificityIsoform 1 is expressed in many tissues. Not found in heart, liver, spleen and testis. Relatively highly expressed in fetal brain. Isoform LMX1A-4B is expressed in testis.
Sequence similaritiesContains 1 homeobox DNA-binding domain.
Contains 2 LIM zinc-binding domains.
- Information by UniProt
- HGNC:6653 antibody
- LIM homeobox protein 1.1 antibody
- LIM homeobox transcription factor 1 alpha antibody
All lanes : Anti-LMX1A antibody (ab82833) at 1/1000 dilution
Lane 1 : uninduced culture of E.coli (one shot Top10 competent cells)
Lane 2 : induced culture of E.coli (one shot Top10 competent cells)
All lanes : anti-Rabbit IgG HRP at 1/1000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa
Predicted molecular weight of protein is 43kDa, tag is 14kDa.
ab82833 has not yet been referenced specifically in any publications.