Overview

  • Product name

    Anti-LMX1A antibody - C-terminal
    See all LMX1A primary antibodies
  • Description

    Rabbit polyclonal to LMX1A - C-terminal
  • Host species

    Rabbit
  • Specificity

    At least two isoforms are known to exist. ab139726 is predicted to not cross-react with LMX1B.
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human LMX1A aa 300-350 (C terminal). The exact sequence is proprietary. 16 amino acids. NP_796372
    Database link: NP_796372

  • Positive control

    • WB: Rat brain tissue lysate. IHC-P: Mouse and human brain tissue. ICC/IF: Mouse neural stem cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab139726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 42 kDa.
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 2.5 µg/ml.

Target

  • Function

    Acts as a transcriptional activator by binding to an A/T-rich sequence, the FLAT element, in the insulin gene promoter. Required for development of the roof plate and, in turn, for specification of dorsal cell fates in the CNS and developing vertebrae.
  • Tissue specificity

    Isoform 1 is expressed in many tissues. Not found in heart, liver, spleen and testis. Relatively highly expressed in fetal brain. Isoform LMX1A-4B is expressed in testis.
  • Sequence similarities

    Contains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • HGNC:6653 antibody
    • LIM homeobox protein 1.1 antibody
    • LIM homeobox transcription factor 1 alpha antibody
    • LIM homeobox transcription factor 1-alpha antibody
    • LIM/homeobox protein 1.1 antibody
    • LIM/homeobox protein LMX1A antibody
    • LMX 1 antibody
    • LMX 1.1 antibody
    • LMX 1A antibody
    • LMX-1.1 antibody
    • LMX1 antibody
    • LMX1.1 antibody
    • LMX1A antibody
    • LMX1A_HUMAN antibody
    • MGC87616 antibody
    see all

Images

  • ab139726 staining LMX1A (red) in mouse neural stem cells by ICC/IF (Immunocytochemistry/immunofluorescence).

    Cells were fixed with formaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 3% BSA for 1 hour at 20°C. Samples were incubated with primary antibody (1/500 in PBS + 3% BSA + 0.3% Triton X-100) for 16 hours at 4°C. An Alexa-Fluor® 568-conjugated donkey anti-rabbit IgG (H+L) monoclonal was used as the secondary antibody.

    Blue - DAPI, Green - Nestin.

    See Abreview

  • Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse brain tissue labeling LMX1A with ab139726 at 5 μg/ml.

  • Lane 1 : Anti-LMX1A antibody - C-terminal (ab139726) at 1 µg
    Lane 2 : Anti-LMX1A antibody - C-terminal (ab139726) at 2 µg

    All lanes : Rat brain tissue lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 42 kDa

  • Immunohistochemistry (Formalin-fixed paraffin embedded sections) of human brain tissue labeling LMX1A with antibody ab139726 at 2.5 μg/ml.

  • Immunofluorescence of human brain tissue labeling LMX1A with ab139726 at 20 μg/ml.

References

This product has been referenced in:

  • Raina A  et al. Neuronal Trans-differentiation by Transcription Factors Ascl1 and Nurr1: Induction of a Dopaminergic Neurotransmitter Phenotype in Cortical GABAergic Neurons. Mol Neurobiol N/A:N/A (2019). Read more (PubMed: 31317490) »
  • Shall G  et al. Effects of Passage Number and Differentiation Protocol on the Generation of Dopaminergic Neurons from Rat Bone Marrow-Derived Mesenchymal Stem Cells. Int J Mol Sci 19:N/A (2018). ICC/IF ; Rat . Read more (PubMed: 29498713) »
See all 4 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Sample
Mouse Cell (Neural stem cells)
Specification
Neural stem cells
Permeabilization
Yes - 0.3% triton
Fixative
Formaldehyde

Dr. Sébastien Brot

Verified customer

Submitted Apr 09 2015

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