Overview

  • Product name

  • Description

    Goat polyclonal to LMX1b
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Hamster, Dog, Monkey, Zebrafish, Opossum, Elephant
  • Immunogen

    Synthetic peptide:

    QQALTTSCYFRDRK

    , corresponding to internal sequence amino acids 64-77 of Human LMX1b (NP_002307.1).

  • Positive control

    • Human testis tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab129306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Preliminary experiments gave an approx 100kDa band in Mouse Kidney lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.6kDa according to Human NP_002307.1 and to Mouse NP_034855.1. The 100kDa band was successfully blocked by incubation with the immunizing peptide.
IHC-P Use a concentration of 2.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Essential for the specification of dorsal limb fate at both the zeugopodal and autopodal levels.
  • Tissue specificity

    Expressed in most tissues. Highest levels in testis, thyroid, duodenum, skeletal muscle, and pancreatic islets.
  • Involvement in disease

    Defects in LMX1B are the cause of nail-patella syndrome (NPS) [MIM:161200]; also known as onychoosteodysplasia. NPS is a disease that cause abnormal skeletal patterning and renal dysplasia.
  • Sequence similarities

    Contains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • LIM homeo box transcription factor 1 beta antibody
    • LIM homeobox transcription factor 1 beta antibody
    • LIM homeobox transcription factor 1-beta antibody
    • LIM-homeobox protein 1.2 antibody
    • LIM/homeobox protein 1.2 antibody
    • LIM/homeobox protein LMX1B antibody
    • LMX 1.2 antibody
    • LMX-1.2 antibody
    • LMX1.2 antibody
    • LMX1B antibody
    • LMX1B_HUMAN antibody
    • MGC138325 antibody
    • MGC142051 antibody
    • NPS 1 antibody
    • NPS1 antibody
    see all

Images

  • ab129306 at 3.75 µg/ml, staining LMX1b in Formalin-Fixed, Paraffin-embedded Human testis tissue by Immunohistochemistry.

References

ab129306 has not yet been referenced specifically in any publications.

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