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r suggestions you made.
With regard to these suggestions, all lysates were made from cells at 80-90% confluency, the lysis buffer was kept at 4C and the samples were boiled. In addition, although a no primary experiment was notperformed, this secondary has been used with another antibody and these very clear bands at 30kDa were not seen.
As it seems this antibody has unspecific binding we were wondering whether you would be able to send us a sample vial of another one of your LMX1B antibodies to test.
Many thanks for your help
Asked on May 28 2012