Immunohistochemistry (Frozen sections) abreview for Anti-LMX1b antibody

Poor
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Embryonic brain)
Specification
Embryonic brain
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/500
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% NDS, 0.1% Triton-X, PBS

Secondary antibody

Name
Non-Abcam antibody was used: Anti-rabbit IgG (H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/250

Additional data

Additional Notes
I tried multiple conditions (0.2% and 4% PFA, with and without antigen retrieval, 1:50 and 1:500 dilution of primary antibody, blocking with milk and serum) -- all gave high background levels.
Abcam response
Thank you for your honest feedback. This antibody has not been tested to work in IHC-Fr, so it may in fact be unsuitable for this application.

Abcam user community

Verified customer

Submitted May 26 2009

Sign up