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Immunohistochemistry (Frozen sections)
Mouse Tissue sections (Embryonic brain)
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% NDS, 0.1% Triton-X, PBS
Non-Abcam antibody was used: Anti-rabbit IgG (H+L)
Host species: Donkey
Conjugation: Alexa Fluor® 488
I tried multiple conditions (0.2% and 4% PFA, with and without antigen retrieval, 1:50 and 1:500 dilution of primary antibody, blocking with milk and serum) -- all gave high background levels.
Thank you for your honest feedback. This antibody has not been tested to work in IHC-Fr, so it may in fact be unsuitable for this application.
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Submitted May 26 2009