Immunohistochemistry (Frozen sections) abreview for Anti-LMX1b antibody

Good
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Zebrafish Tissue sections (heart, fin)
Specification
heart, fin
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Tween 20 in PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 37°C

Other product details

Dilution
1/100
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 37°C

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor 488/594
Host species: Goat
Clonality: Polyclonal
Conjugation: Other
Dilution
1/200

Additional data

Additional Notes
Can't comment on specificity as antibody was not tested in a tissue where lmx1b is not expressed.

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Submitted Jul 05 2010

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